Costion, C.Lowe, A.Rossetto, M.Kooyman, R.Breed, M.Ford, A.Crayn, D.2018-11-152018-11-152016Diversity, 2016; 8(1):5-1-5-91424-28181424-2818http://hdl.handle.net/2440/116082A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland’s Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research.en© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).Biogeography; wet tropics; rbcLa; matK; trnH-psbA intergenic spacerJournal article003004540210.3390/d80100050003740493000042-s2.0-84966349180237369Lowe, A. [0000-0003-1139-2516]Breed, M. [0000-0001-7810-9696]