Lu, L.Qiu, W.Gao, W.Tyerman, S.Shou, H.Wang, C.2017-03-142017-03-142016Plant, Cell and Environment, 2016; 39(10):2247-22590140-77911365-3040http://hdl.handle.net/2440/103813Under phosphate (Pi ) starvation, plants increase the secretion of purple acid phosphatases (PAPs) into the rhizosphere to scavenge organic phosphorus (P) for plant use. To date, only a few members of the PAP family have been characterized in crops. In this study, we identified a novel secreted PAP in rice, OsPAP10c, and investigated its role in the utilization of external organic P. OsPAP10c belongs to a monocotyledon-specific subclass of Ia group PAPs and is specifically expressed in the epidermis/exodermis cell layers of roots. Both the transcript and protein levels of OsPAP10c are strongly induced by Pi starvation. OsPAP10c overexpression increased acid phosphatase (APase) activity by more than 10-fold in the culture media and almost fivefold in both roots and leaves under Pi -sufficient and Pi -deficient conditions. This increase in APase activity further improved the plant utilization efficiency of external organic P. Moreover, several APase isoforms corresponding to OsPAP10c were identified using in-gel activity assays. Under field conditions with three different Pi supply levels, OsPAP10c-overexpressing plants had significantly higher tiller numbers and shorter plant heights. This study indicates that OsPAP10c encodes a novel secreted APase that plays an important role in the utilization of external organic P in rice.en© 2016 John Wiley & Sons LtdOryza sativa (rice)purple acid phosphatase (PAP)Journal article003005065410.1111/pce.127940003858461000112-s2.0-849828929082-s2.0-84980649440257733Tyerman, S. [0000-0003-2455-1643]