Nefzger, C.M.Jardé, T.Srivastava, A.Schroeder, J.Rossello, F.J.Horvay, K.Prasko, M.Paynter, J.M.Chen, J.Weng, C.-F.Sun, Y.B.Y.Liu, X.Chan, E.Deshpande, N.Chen, X.Li, Y.J.Pflueger, J.Engel, R.M.Knaupp, A.S.Tsyganov, K.et al.2022-06-282022-06-282022npj Regenerative Medicine, 2022; 7(1):31-1-31-82057-39952057-3995https://hdl.handle.net/2440/135652The impact of aging on intestinal stem cells (ISCs) has not been fully elucidated. In this study, we identified widespread epigenetic and transcriptional alterations in old ISCs. Using a reprogramming algorithm, we identified a set of key transcription factors (Egr1, Irf1, FosB) that drives molecular and functional differences between old and young states. Overall, by dissecting the molecular signature of aged ISCs, our study identified transcription factors that enhance the regenerative capacity of ISCs.en© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons. org/licenses/by/4.0/.Journal article10.1038/s41536-022-00226-72022-06-28615158Polo, J.M. [0000-0002-2531-778X]