Hoefel, D.Adriansen, C.Bouyssou, M.Saint, C.Newcombe, G.Ho, L.2010-03-302010-03-302009Applied and Environmental Microbiology, 2009; 75(15):5167-51690099-22401098-5336http://hdl.handle.net/2440/57201Copyright © 2009, American Society for Microbiology. All Rights Reserved.We report for the first time a quantitative mlrA gene-directed TaqMan PCR assay for the rapid detection of microcystin-degrading bacteria. This was applied, in combination with 16S ribosomal DNA-directed quantitative PCR and denaturing gradient gel electrophoresis, to study virgin sand filter column biofilm development and to correlate mlrA gene abundance with microcystin removal efficiency.enBacteriaBiofilmsBacterial ProteinsDNA, BacterialDNA, RibosomalRNA, Ribosomal, 16SElectrophoresis, Polyacrylamide GelColony Count, MicrobialCluster AnalysisDNA FingerprintingPolymerase Chain ReactionSequence Analysis, DNASoil MicrobiologyWater PurificationNucleic Acid DenaturationMolecular Sequence DataMicrocystinsDevelopment of an mlrA gene-directed TaqMan PCR assay for quantitative assessment of microcystin-degrading bacteria within water treatment plant sand filter biofilmsJournal article002009153510.1128/AEM.00036-090002683116000312-s2.0-6765124759438267