Somers, W.Saint, R.2006-06-192006-06-192003Developmental Cell, 2003; 4(1):29-391534-58071878-1551http://hdl.handle.net/2440/2970The mechanism that positions the cytokinetic contractile ring is unknown, but derives from the spindle midzone. We show that an interaction between the Rho GTP exchange factor, Pebble, and the Rho family GTPase-activating protein, RacGAP50C, connects the contractile ring to cortical microtubules at the site of furrowing in D. melanogaster cells. Pebble regulates actomyosin organization, while RacGAP50C and its binding partner, the Pavarotti kinesin-like protein, regulate microtubule bundling. All three factors are required for cytokinesis. As furrowing begins, these proteins colocalize to a cortical equatorial ring. We propose that RacGAP50C-Pavarotti complexes travel on cortical microtubules to the cell equator, where they associate with the Pebble RhoGEF to position contractile ring formation and coordinate F-actin and microtubule remodeling during cytokinesis.enMicrotubulesAnimalsDrosophila melanogasterMacromolecular SubstancesGTPase-Activating ProteinsGuanine Nucleotide Exchange FactorsMicrotubule-Associated ProteinsDrosophila ProteinsRNA, MessengerTwo-Hybrid System TechniquesCell DivisionProtein BindingRho Guanine Nucleotide Exchange FactorsSpindle ApparatusWings, AnimalJournal article002003033910.1016/S1534-5807(02)00402-10001808620000082-s2.0-003724435258884