Neumann, D.P.Pillman, K.A.Dredge, B.K.Bert, A.G.Phillips, C.A.Lumb, R.Ramani, Y.Bracken, C.P.Hollier, B.G.Selth, L.A.Beilharz, T.H.Goodall, G.J.Gregory, P.A.2024-08-192024-08-192024RNA Biology, 2024; 21(1):1-111547-62861555-8584https://hdl.handle.net/2440/141932Published online: 19 Dec 2023Epithelial-mesenchymal transition (EMT) plays important roles in tumour progression and is orchestrated by dynamic changes in gene expression. While it is well established that post-transcriptional regulation plays a significant role in EMT, the extent of alternative polyadenylation (APA) during EMT has not yet been explored. Using 3' end anchored RNA sequencing, we mapped the alternative polyadenylation (APA) landscape following Transforming Growth Factor (TGF)-β-mediated induction of EMT in human mammary epithelial cells and found APA generally causes 3'UTR lengthening during this cell state transition. Investigation of potential mediators of APA indicated the RNA-binding protein Quaking (QKI), a splicing factor induced during EMT, regulates a subset of events including the length of its own transcript. Analysis of QKI crosslinked immunoprecipitation (CLIP)-sequencing data identified the binding of QKI within 3' untranslated regions (UTRs) was enriched near cleavage and polyadenylation sites. Following QKI knockdown, APA of many transcripts is altered to produce predominantly shorter 3'UTRs associated with reduced gene expression. These findings reveal the changes in APA that occur during EMT and identify a potential role for QKI in this process.en© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.3’ untranslated region (3’UTR)alternative polyadenylationCrosslinked immunopreciptation (CLIP) sequencingepithelial-mesenchymal transitionQuakingRNA binding protein (RBP)HumansRNA-Binding Proteins3' Untranslated RegionsGene Expression RegulationPolyadenylationBase SequenceEpithelial-Mesenchymal TransitionJournal article10.1080/15476286.2023.22942222024-08-15679382Pillman, K.A. [0000-0002-5869-889X]Dredge, B.K. [0000-0002-6103-5165]Selth, L.A. [0000-0002-4686-1418]Goodall, G.J. [0000-0003-1294-0692]Gregory, P.A. [0000-0002-0999-0632]