Akter, J.Hayball, E.F.Bell, S.G.2024-07-292024-07-292023Catalysis Science & Technology, 2023; 13(22):6355-63592044-47532044-4761https://hdl.handle.net/2440/141709A crucial reaction in chemical synthesis is the activation of unactivated carbon–hydrogen bonds in complex molecules. We demonstrate the regio- and stereo-selective hydroxlation of the steroids progesterone and androstenedione using the peroxygenase activity of an engineered bacterial cytochrome P450 enzyme, CYP154C8. By replacing a single amino acid of the I-helix we change this monooxygenase enzyme into a peroxygenase, enabling the efficient and selective biocatalytic formation of the 16α-hydroxy steroid metabolite.enThis journal is © The Royal Society of Chemistry 2023carbon-hydrogen; moleculesEfficient regio- and stereo-selective C-H bond hydroxylation of steroids using an engineered heme-thiolate peroxygenase biocatalystJournal article10.1039/d3cy01223a2024-07-26662536Akter, J. [0000-0003-1798-474X]Hayball, E.F. [0000-0001-9026-8630]Bell, S.G. [0000-0002-7457-9727]