Sallustio, B.Fairchild, B.2006-07-262006-07-261995Journal of Chromatography A, 1995; 665(2):345-3530021-96731879-1794http://hdl.handle.net/2440/14460Gemfibrozil 1-O-β-acylglucuronide was purified from the urine of a volunteer administered gemfibrozil, and an isocratic reversed-phase HPLC method was developed for its direct measurement. Quantitation of gemfibrozil and gemfibrozil 1-O-β-acylglucuronide was carried out from plasma, following extraction from acidified specimens into ethyl acetate, on a 5-μm CN reversed-phase column with a mobile phase (pH 3.5) containing acetonitrile, tetrabutylammonium sulphate and distilled water, using fluorescence detection at 284 nm excitation and 316 nm emission. Calibration curves were linear for both compounds over a concentration range of 0.1 to 40 mg/l, with intra-assay coefficients of variation <5% at concentrations of 20.0, 2.0 and 0.2 mg/l, and inter-assay coefficients of variation <10%. No degradation of gemfibrozil 1-O-β-acylglucuronide was detected as a result of the analytical procedure. However, a preliminary application of the method indicates that gemfibrozil acylglucuronide is chemically unstable undergoing intra-molecular rearrangement and hydrolysis under physiological conditions.enCopyright © 1995 Published by Elsevier B.V.HumansGemfibrozilGlucuronatesChromatography, High Pressure LiquidChromatography, Thin LayerMagnetic Resonance SpectroscopyDrug StabilityHydrogen-Ion ConcentrationQuality ControlMass SpectrometryJournal article0030003400001995052010.1016/0378-4347(94)00530-IA1995QQ434000092-s2.0-002895677267406Sallustio, B. [0000-0002-0186-3073]