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https://hdl.handle.net/2440/11265
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dc.contributor.author | Jitrapakdee, S. | - |
dc.contributor.author | Walker, M. | - |
dc.contributor.author | Wallace, J. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, 1996; 223(3):695-700 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11265 | - |
dc.description.abstract | We have identified and characterized multiple mRNA transcripts of rat and human pyruvate carboxylases [EC 6.4.1.1] using rapid amplification of cDNA 5' ends-polymerase chain reaction (RACE-PCR). Five alternative forms of rat pyruvate carboxylase cDNAs have been identified in liver, kidney, brain, and adipose tissue and these are expressed in a tissue-specific manner. Two alternative forms of human pyruvate carboxylase cDNA have also been identified in liver. These pyruvate carboxylase cDNAs have a common coding region but differ in their 5' untranslated regions (5'UTRs), suggesting that they are generated by alternative splicing of the primary transcript. Southern blot analysis of restriction enzyme digested rat genomic DNA revealed that pyruvate carboxylase is encoded by a single copy gene. | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | - |
dc.source.uri | http://dx.doi.org/10.1006/bbrc.1996.0958 | - |
dc.subject | Kidney | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Rats | - |
dc.subject | Isoenzymes | - |
dc.subject | Pyruvate Carboxylase | - |
dc.subject | Recombinant Proteins | - |
dc.subject | RNA, Messenger | - |
dc.subject | DNA Primers | - |
dc.subject | Cloning, Molecular | - |
dc.subject | Random Amplified Polymorphic DNA Technique | - |
dc.subject | Protein Biosynthesis | - |
dc.subject | Alternative Splicing | - |
dc.subject | Base Sequence | - |
dc.subject | Regulatory Sequences, Nucleic Acid | - |
dc.subject | Sequence Homology, Nucleic Acid | - |
dc.subject | Molecular Sequence Data | - |
dc.title | Identification of Novel Alternatively Spliced Pyruvate Carboxylase mRNAs with Divergent 5' -Untranslated Regions Which Are Expressed in a Tissue-Specific Manner | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1006/bbrc.1996.0958 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Walker, M. [0000-0002-6934-3787] | - |
Appears in Collections: | Aurora harvest 2 Biochemistry publications |
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