Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/57279
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Immunomodulatory effect of cytosine-phosphate-guanosine (CpG)-oligonucleotides in nonasthmatic chronic rhinosinusitis: An explant model |
Author: | Tan, L. Rogers, T. Hatzirodos, N. Baker, L. Ooi, E. Wormald, P. |
Citation: | American Journal of Rhinology and Allergy, 2009; 23(2):123-129 |
Publisher: | Ocean Side Publications Inc |
Issue Date: | 2009 |
ISSN: | 1945-8924 1945-8932 |
Statement of Responsibility: | Lorwai Tan, Trisha J Rogers, Nick Hatzirodos, Leonie M Baker, Enghooi Ooi and Peter-John Wormald |
Abstract: | BACKGROUND: The use of cytosine-phosphate-guanosine-oligodeoxynucleotides (CpG-ODNs) or immunostimulatory sequences (ISSs) in the treatment of airway diseases is gaining interest. Binding of the CpG-ODN ligand to Toll-like receptor 9 (TLR9) triggers a shift from a Th2- to a Th1-type response in the target tissue. In this study, we explored the potential use of CpG-ODN to dampen the predominantly Th2-driven chronic inflammatory state in our cohort of patients. METHODS: An in vitro explant model comprising of sinonasal tissue from patients with asthma (n = 12) and without asthma (n = 11) were stimulated with CpG-ODN or Staphylococcus aureus enterotoxin B (SEB) or CpG-ODN in combination with SEB for 48 hours. Ten of the 12 asthma patients had nasal polyps. RNA was extracted for multiplex real-time reverse transcription polymerase chain reaction analysis and the 2(-delta deltaC(T)) method used to determine interleukin (IL)-5, p35 IL-12, interferon (IFN) gamma, and TLR9 expression levels. RESULTS: CpG-ODN significantly reduced IL-5 mRNA expression in patients without asthma (p = 0.0379) but not in the asthma-associated group. SEB alone caused an increase in IL-5 levels that could be dampened when CpG-ODN was added in combination with SEB. Significant differences in mean IL-5 expression levels between the asthmatic and nonasthmatic categories were detected (Welch t-test; **p = 0.0041). Asthmatic and nonasthmatic patients present as two distinct categories as reflected by significant differences in their IL-5 response to CpG-ODN (F = 11.93; ***p = 0.0008), SEB (F = 41.34; *p = 0.0476) and CpG-ODN with SEB (F = 13.2; *p = 0.0114). In contrast, no significant differences were observed in the expression levels of IL-12, IFN-gamma, and TLR9. CONCLUSION: Localized application of CpG-ODN on its own or in combination with SEB may potentially reduce the expression of the proinflammatory cytokine IL-5 in nonasthmatic patients and may be further developed as an immunotherapeutic agent. |
Keywords: | Adult Aged 80 and over Asthma Cohort Studies Enterotoxins Female Gene Expression Regulation Humans Immunologic Factors Interleukin-5 Male Middle Aged Nasal Polyps Oligodeoxyribonucleotides Paranasal Sinuses Respiratory Mucosa Rhinitis Sinusitis Staphylococcus aureus Tissue Culture Techniques Toll-Like Receptor 9 immunology pathology physiopathology metabolism genetics |
DOI: | 10.2500/ajra.2009.23.3279 |
Description (link): | http://pubget.com/paper/19401034?title=Immunomodulatory+effect+of+cytosine-phosphate-guanosine+%28CpG%29-oligonucleotides+in+nonasthmatic+chronic+rhinosinusitis%3A+an+explant+model. |
Published version: | http://dx.doi.org/10.2500/ajra.2009.23.3279 |
Appears in Collections: | Aurora harvest Surgery publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.