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https://hdl.handle.net/2440/70078
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Type: | Journal article |
Title: | Increased T regulatory cells and decreased Th1 pro-inflammatory cytokines correlate with culture-positive infection in febrile neutropenia childhood oncology patients |
Author: | Hodge, G. Scott, J. Osborn, M. To, L. Zola, H. Hodge, S. Revesz, T. |
Citation: | Cytokine, 2011; 53(3):286-288 |
Publisher: | Academic Press |
Issue Date: | 2011 |
ISSN: | 1096-0023 1043-4666 |
Statement of Responsibility: | G. Hodge, J. Scott, M. Osborn, L.B. To, H. Zola, S. Hodge, T. Revesz |
Abstract: | <h4>Background</h4>Paediatric oncology patients with febrile neutropenia are usually hospitalised and treated with empirical broad-spectrum antibiotic therapy to counter the risk of infection. However, there is currently no method available to rapidly identify bacteremia in these patients. T-helper-type-1 (Th1) cytokines are required for effective immune response to many pathogenic organisms and T regulatory cells are known suppressors of Th1 cells. We hypothesized that characterization of reduced intracellular Th1 cytokines and increased T regulatory cells (Tregs) may prove useful in identifying infection in childhood oncology patients with febrile neutropenia.<h4>Methods</h4>Intracellular Th 1 cytokines and Tregs were enumerated in peripheral blood from a group of childhood oncology patients with febrile neutropenia using multiparameter flow cytometry.<h4>Results</h4>There was a significant increase in the percentage of CD25(+) CD127(-) CD8(-) CD3(+) Tregs and a significant decrease in Th1 intracellular cytokines IFNγ, IL-2 and TNFα in the blood of culture positive patients compared with culture negative patients.<h4>Conclusions</h4>Enumeration of Tregs and intracellular Th1 cytokines may provide a sensitive, specific test for determining infection in childhood oncology patients before blood culture results become available. |
Keywords: | Febrile neutropenia Infection T regulatory cells Th1 cytokines |
Rights: | Crown copyright © 2010 Published by Elsevier Ltd. All rights reserved. |
DOI: | 10.1016/j.cyto.2010.11.009 |
Published version: | http://dx.doi.org/10.1016/j.cyto.2010.11.009 |
Appears in Collections: | Aurora harvest Paediatrics publications |
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