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Type: | Journal article |
Title: | Transcript abundance of amino acid transporters, β-casein, and α-lactalbumin in mammary tissue of periparturient, lactating, and postweaned sows |
Other Titles: | Transcript abundance of amino acid transporters, beta-casein, and alpha-lactalbumin in mammary tissue of periparturient, lactating, and postweaned sows |
Author: | Manjarin, R. Steibel, J. Zamora, V. Am-in, N. Kirkwood, R. Ernst, C. Weber, P. Taylor, N. Trottier, N. |
Citation: | Journal of Dairy Science, 2011; 94(7):3467-3476 |
Publisher: | Elsevier |
Issue Date: | 2011 |
ISSN: | 0022-0302 1525-3198 |
Statement of Responsibility: | R. Manjarin J. P. Steibel V. Zamora N. Am-in R. N. Kirkwood C. W. Ernst P. S. Weber N. P. Taylor and N. L. Trottier |
Abstract: | The objective of these experiments was to test the hypothesis that transcript abundance of cationic AA transporter- and milk protein-encoding genes increase in the porcine mammary gland in response to higher lactation demand. Genes of interest included those encoding for the milk proteins α-lactalbumin (α-LA) and β-casein (β-CN; LALBA and CSN2, respectively), and AA transporter b(0,+)AT, y(+)LAT1, y(+)LAT2, ATB(0,+), CAT-1, and CAT-2b (SLC7A9, SLC7A7, SLC7A6, SLC6A14, SLC7A1, and SLC7A2, respectively). Mammary tissue was biopsied from 4 sows on d 110 of gestation (prepartum), on d 2 (early postpartum), on d 5 (early), and d 17 (peak) of lactation, and on d 5 after weaning (postweaning), and mRNA of target genes quantified by reverse transcription quantitative PCR. Compared with prepartum, CAT-1, ATB(0,+), y(+)LAT2, β-CN, and α-LA mRNA abundance was higher at early lactation, whereas compared with early lactation, only CAT-1 and α-LA mRNA abundance was higher at peak lactation. The CAT-2b, y(+)LAT1, and b(0,+)AT mRNA abundance did not differ when comparing either prepartum or peak lactation to early lactation. Compared with peak lactation, postweaning mRNA abundance of CAT-1, ATB(0,+), α-LA, and β-CN decreased, y(+)LAT2, CAT-2b, and b(0,+)AT remained unchanged, and y(+)LAT1 increased. The mRNA abundance of y(+)LAT2 increased from early postpartum to early lactation, and remained unchanged for CAT-1, ATB(0,+), α-LA, and β-CN. From prepartum to peak lactation, the mRNA abundance of CAT-1, y(+)LAT2, and ATB(0,+) was positively correlated with that of β-CN and α-LA. In conclusion, the expression of genes encoding for y(+)LAT1, CAT-2b, and b(0,+)AT remained unchanged in porcine mammary glands over prepartum to peak lactation period, whereas expression of genes encoding for CAT-1, ATB(0,+), and y(+)LAT2 was upregulated and positively correlated to expression of genes encoding for the mammary synthesized milk proteins β-CN and α-LA. |
Keywords: | Mammary Glands, Animal Animals Swine Lactalbumin Amino Acid Transport Systems Caseins RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Weaning Lactation Female Peripartum Period |
Rights: | © American Dairy Science Association®, 2011 |
DOI: | 10.3168/jds.2011-4163 |
Published version: | http://dx.doi.org/10.3168/jds.2011-4163 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 7 |
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