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https://hdl.handle.net/2440/87492
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Type: | Book chapter |
Title: | Imaging blood vessels in the zebrafish |
Author: | Kamei, M. Isogai, S. Pan, W. Weinstein, B.M. |
Citation: | The Zebrafish: Cellular and Developmental Biology, Part A, 2010 / Detrich, H.W., Westerfield, M., Zon, L.I. (ed./s), vol.100, Ch.2, pp.27-54 |
Publisher: | Elsevier |
Issue Date: | 2010 |
Series/Report no.: | Methods in Cell Biology; 100 |
ISBN: | 9780123848925 |
Editor: | Detrich, H.W. Westerfield, M. Zon, L.I. |
Statement of Responsibility: | Makoto Kamei, Sumio Isogai, Weijun Pan, and Brant M. Weinstein |
Abstract: | Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines. |
Keywords: | Blood Vessels Embryo, Nonmammalian Animals Animals, Genetically Modified Zebrafish Angiography In Situ Hybridization Time-Lapse Imaging |
Rights: | Copyright © 2010 Elsevier Inc. All rights reserved. |
DOI: | 10.1016/B978-0-12-384892-5.00002-5 |
Published version: | http://dx.doi.org/10.1016/b978-0-12-384892-5.00002-5 |
Appears in Collections: | Aurora harvest 7 Paediatrics publications |
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