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https://hdl.handle.net/2440/100161
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dc.contributor.author | Babb, R. | - |
dc.contributor.author | Chen, A. | - |
dc.contributor.author | Hirst, T. | - |
dc.contributor.author | Kara, E. | - |
dc.contributor.author | McColl, S. | - |
dc.contributor.author | Ogunniyi, A. | - |
dc.contributor.author | Paton, J. | - |
dc.contributor.author | Alsharifi, M. | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Clinical Science, 2016; 130(9):697-710 | - |
dc.identifier.issn | 0143-5221 | - |
dc.identifier.issn | 1470-8736 | - |
dc.identifier.uri | http://hdl.handle.net/2440/100161 | - |
dc.description.abstract | Generating a pneumococcal vaccine that is serotype independent and cost effective remains a global challenge. γ-Irradiation has been used widely to sterilize biological products. It can also be utilized as an inactivation technique to generate whole-cell bacterial and viral vaccines with minimal impact on pathogen structure and antigenic determinants. In the present study, we utilized γ-irradiation to inactivate an un-encapsulated Streptococcus pneumoniae strain Rx1 with an unmarked deletion of the autolysin gene lytA and with the pneumolysin gene ply replaced with an allele encoding a non-toxic pneumolysoid (PdT) (designated γ-PN vaccine). Intranasal vaccination of C57BL/6 mice with γ-PN was shown to elicit serotype-independent protection in lethal challenge models of pneumococcal pneumonia and sepsis. Vaccine efficacy was shown to be reliant on B-cells and interleukin (IL)-17A responses. Interestingly, immunization promoted IL-17 production by innate cells not T helper 17 (Th17) cells. These data are the first to report the development of a non-adjuvanted intranasal γ-irradiated pneumococcal vaccine that generates effective serotype-independent protection, which is mediated by both humoral and innate IL-17 responses. | - |
dc.description.statementofresponsibility | Rachelle Babb, Austen Chen, Timothy R. Hirst, Ervin E. Kara, Shaun R. McColl, Abiodun D. Ogunniyi, James C. Paton, and Mohammed Alsharifi | - |
dc.language.iso | en | - |
dc.publisher | Portland Press | - |
dc.rights | © 2016 Authors; published by Portland Press Limited | - |
dc.source.uri | http://dx.doi.org/10.1042/cs20150699 | - |
dc.subject | Protective immunity; serotype independent; Streptococcus pneumoniae; vaccine | - |
dc.title | Intranasal vaccination with γ -irradiated Streptococcus pneumoniae whole-cell vaccine provides serotype-independent protection mediated by B-cells and innate IL-17 responses | - |
dc.title.alternative | Intranasal vaccination with gamma -irradiated Streptococcus pneumoniae whole-cell vaccine provides serotype-independent protection mediated by B-cells and innate IL-17 responses | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1042/CS20150699 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/LP120200244 | - |
dc.relation.grant | NHMRC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McColl, S. [0000-0003-0949-4660] | - |
dc.identifier.orcid | Ogunniyi, A. [0000-0001-9308-5629] | - |
dc.identifier.orcid | Paton, J. [0000-0001-9807-5278] | - |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
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