Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/10359
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Dipeptidase 1: a candidate tumor-specific molecular marker in colorectal carcinoma |
Author: | McIver, C. Lloyd, J. Hewett, P. Hardingham, J. |
Citation: | Cancer Letters, 2004; 209(1):67-74 |
Publisher: | Elsevier Sci Ireland Ltd |
Issue Date: | 2004 |
ISSN: | 0304-3835 1872-7980 |
Abstract: | The aim of this study was to identify tumor-specific markers for the detection of rare disseminated colorectal tumor cells in peripheral venous blood and in intra-peritoneal saline lavage samples collected before and after resection of colorectal tumors. Using cDNA micro-array screening, we found dipeptidase 1 (DPEP1) to be highly expressed in colon tumors compared to matched normal mucosa. Relative reverse transcriptase (RT)-PCR showed that DPEP1 was over-expressed by >/=2 fold in colon tumor compared to normal colonic mucosal tissue in 56/68 (82%) patients. Using immunobead RT-PCR, a technique that first enriches for epithelial cells, we found DPEP1 positive cells in intra-peritoneal lavage and venous blood samples from 15/38 (39%) colorectal cancer cases. This is the first report of DPEP1 as a marker for disseminated colon tumor cells. |
Keywords: | Cell Line, Tumor Epithelial Cells Humans Colorectal Neoplasms Neoplasm Metastasis Dipeptidases DNA, Complementary Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Adult Aged Aged, 80 and over Middle Aged Female Male GPI-Linked Proteins Biomarkers, Tumor |
DOI: | 10.1016/j.canlet.2003.11.033 |
Published version: | http://dx.doi.org/10.1016/j.canlet.2003.11.033 |
Appears in Collections: | Aurora harvest 2 Surgery publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.