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https://hdl.handle.net/2440/112020
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dc.contributor.author | Yoo, A. | - |
dc.contributor.author | Rossi-Fedele, G. | - |
dc.contributor.author | Kidd, S. | - |
dc.contributor.author | Rogers, A. | - |
dc.contributor.author | Zilm, P. | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Journal of Endodontics, 2018; 44(2):269-273 | - |
dc.identifier.issn | 0099-2399 | - |
dc.identifier.issn | 1878-3554 | - |
dc.identifier.uri | http://hdl.handle.net/2440/112020 | - |
dc.description.abstract | Extracellular material (ECM) surrounding Enterococcus faecalis may play a role in increasing resistance to environmental stresses. Our aim was to determine ECM levels in response to subminimal inhibitory concentrations of sodium hypochlorite (sub-MIC/NaOCl) or anaerobic growth and determine the impact on biofilm development.From 37 E. faecalis clinical strains, 19 were selected according to their biofilm-producing ability by using a crystal violet biofilm assay: 10 strong, 4 intermediate, and 5 non-biofilm producers. Biofilm assays were subsequently performed on all strains when subjected to sub-MIC/NaOCl. All strains were evaluated for ECM production under aerobic and anaerobic conditions and with sub-MIC/NaOCl. ECM production was assessed by using scanning electron microscopy. Double-blinded independent assessors were used to score levels of ECM production. The esp gene was detected by using polymerase chain reaction. Gelatinase activity was determined by using Todd-Hewitt and gelatin agar.In aerobic conditions, ECM was expressed in all strains. In the presence of sub-MIC/NaOCl, of the 10 strong biofilm producers, 5 increased their ECM production, and 4 showed increased biofilm growth. Two strains had less ECM production and showed decreased biofilm growth. One isolate demonstrated no observable changes. Most non-biofilm producers demonstrated no observable differences in ECM production, although 1 strain increased biofilm growth. ECM production in anaerobic conditions was highly variable. The esp gene (n = 15) and gelatinase activity (n = 7) were evident among the isolates.Clonal diversity among strains of E. faecalis suggests that some strong biofilm producers can upregulate ECM production and increase biofilm growth in response to sub-MIC/NaOCl. | - |
dc.description.statementofresponsibility | Austin Yoo, Giampiero Rossi-Fedele, Stephen P. Kidd, Anthony H.Rogers, Peter S. Zilm | - |
dc.language.iso | en | - |
dc.publisher | American Association of Endodontists | - |
dc.rights | © 2017 American Association of Endodontists. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.joen.2017.08.025 | - |
dc.subject | Biofilm | - |
dc.subject | Enterococcus faecalis | - |
dc.subject | clonal diversity | - |
dc.subject | extracellular material | - |
dc.subject | stress response | - |
dc.title | Association between Extracellular Material and Biofilm Formation in Response to Sodium Hypochlorite by Clinical Isolates of Enterococcus faecalis | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.joen.2017.08.025 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Rossi-Fedele, G. [0000-0002-8287-1226] | - |
dc.identifier.orcid | Kidd, S. [0000-0002-2118-1651] | - |
dc.identifier.orcid | Zilm, P. [0000-0001-7554-9717] | - |
Appears in Collections: | Aurora harvest 3 Dentistry publications |
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