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Type: Journal article
Title: Supragingival plaque microbiome ecology and functional potential in the context of health and disease
Author: Espinoza, J.
Harkins, D.
Torralba, M.
Gomez, A.
Highlander, S.
Jones, M.
Leong, P.
Saffery, R.
Bockmann, M.
Kuelbs, C.
Inman, J.
Hughes, T.
Craig, J.
Nelson, K.
Dupont, C.
Citation: mBio, 2018; 9(6):1-21
Publisher: American Society for Microbiology
Issue Date: 2018
ISSN: 2150-7511
Statement of
Josh L. Espinoza, Derek M. Harkins, Manolito Torralba, Andres Gomez, Sarah K. Highlander, Marcus B. Jones, Pamela Leong, Richard Saffery, Michelle Bockmann, Claire Kuelbs, Jason M. Inman, Toby Hughes, Jeffrey M. Craig, Karen E. Nelson, Chris L. Dupont
Abstract: To address the question of how microbial diversity and function in the oral cavities of children relates to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microbiome. Caries phenotypes contained statistically significant enrichments in specific genome abundances and distinct community composition profiles, including strain-level changes. Metabolic pathways that are statistically associated with caries include several sugar-associated phosphotransferase systems, antimicrobial resistance, and metal transport. Numerous closely related previously uncharacterized microbes had substantial variation in central metabolism, including the loss of biosynthetic pathways resulting in auxotrophy, changing the ecological role. We also describe the first complete Gracilibacteria genomes from the human microbiome. Caries is a microbial community metabolic disorder that cannot be described by a single etiology, and our results provide the information needed for next-generation diagnostic tools and therapeutics for caries.
Keywords: Streptococcus; metabolism; metagenomics; microbial ecology; oral microbiology
Rights: © 2018 Espinoza et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
RMID: 0030105244
DOI: 10.1128/mBio.01631-18
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Appears in Collections:Dentistry publications

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