Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/118914
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Full metadata record
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dc.contributor.author | Hao, N. | - |
dc.contributor.author | Shearwin, K.E. | - |
dc.contributor.author | Dodd, I.B. | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Cell Reports, 2019; 26(9):2419-2433.e1–e3 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | http://hdl.handle.net/2440/118914 | - |
dc.description.abstract | Enhancers are ubiquitous and critical gene-regulatory elements. However, quantitative understanding of the role of DNA looping in the regulation of enhancer action and specificity is limited. We used the Escherichia coli NtrC enhancer-σ54 promoter system as an in vivo model, finding that NtrC activation is highly sensitive to the enhancer-promoter (E-P) distance in the 300-6,000 bp range. DNA loops formed by Lac repressor were able to strongly regulate enhancer action either positively or negatively, recapitulating promoter targeting and insulation. A single LacI loop combining targeting and insulation produced a strong shift in specificity for enhancer choice between two σ54 promoters. A combined kinetic-thermodynamic model was used to quantify the effect of DNA-looping interactions on promoter activity and revealed that sensitivity to E-P distance and to control by other loops is itself dependent on enhancer and promoter parameters that may be subject to regulation. | - |
dc.description.statementofresponsibility | Nan Hao, Keith E.Shearwin, Ian B.Dodd | - |
dc.language.iso | en | - |
dc.publisher | Cell Press | - |
dc.rights | © 2019 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | - |
dc.source.uri | http://dx.doi.org/10.1016/j.celrep.2019.02.002 | - |
dc.subject | DNA looping | - |
dc.subject | E. coli | - |
dc.subject | Lac repressor | - |
dc.subject | NtrC | - |
dc.subject | enhancer | - |
dc.subject | insulator | - |
dc.subject | modeling | - |
dc.subject | promoter competition | - |
dc.subject | promoter targeting | - |
dc.subject | sigma-54 promoter | - |
dc.title | Positive and negative control of enhancer-promoter interactions by other DNA loops generates specificity and tunability | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.celrep.2019.02.002 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1100651 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DE150100091 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP160101450 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Hao, N. [0000-0001-5836-3507] | - |
dc.identifier.orcid | Shearwin, K.E. [0000-0002-7736-2742] | - |
dc.identifier.orcid | Dodd, I.B. [0000-0003-2969-6841] | - |
Appears in Collections: | Aurora harvest 8 Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_118914.pdf | Published version | 2.49 MB | Adobe PDF | View/Open |
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