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Type: Journal article
Title: Understanding the mechanistic contribution of herbal extracts in compound Kushen injection with transcriptome analysis
Author: Shen, H.
Qu, Z.
Harata-Lee, Y.
Aung, T.N.
Cui, J.
Wang, W.
Kortschak, D.R.
Adelson, D.L.
Citation: Frontiers in Oncology, 2019; 9(JULY):632-1-632-11
Publisher: Frontiers Media
Issue Date: 2019
ISSN: 2234-943X
Statement of
Hanyuan Shen, Zhipeng Qu, Yuka Harata-Lee, Thazin Nwe Aung, Jian Cui, Wei Wang, R. Daniel Kortschak and David L. Adelson
Abstract: Herbal compatibility is the knowledge of which herbs to combine in traditional Chinese medicine (TCM) formulations. The lack of understanding of herbal compatibility is one of the key problems for the application and popularization of TCM in western society. Because of the chemical complexity of herbal medicines, it is simpler to begin to conduct compatibility research based on herbs rather than component plant secondary metabolites. We have used transcriptome analysis to explore the effects and interactions of two plant extracts (Kushen and Baituling) combined in Compound Kushen Injection (CKI). Based on shared chemical compounds and in vitro cytotoxicity comparisons, we found that both the major compounds in CKI, and the cytotoxicity effects of CKI were mainly derived from the extract of Kushen (Sophorae flavescentis). We generated and analyzed transcriptome data from MDA-MB-231 cells treated with single-herb extracts or CKI and results showed that Kushen contributed to the perturbation of the majority of cytotoxicity/cancer related pathways in CKI such as cell cycle and DNA replication. We also found that Baituling (Heterosmilax yunnanensis Gagnep) could not only enhance the cytotoxic effects of Kushen in CKI, but also activate immune-related pathways. Our analyses predicted that IL-1β gene expression was upregulated by Baituling in CKI and we confirmed that IL-1β protein expression was increased using an ELISA assay. Altogether, these findings help to explain the rationale for combining Kushen and Baituling in CKI, and show that transcriptome analysis using single herb extracts is an effective method for understanding herbal compatibility in TCM.
Keywords: transcriptome
herb combination
traditional Chinese medicine
cancer treatment
Compound Kushen Injection
Description: Published: 12 July 2019
Rights: Copyright © 2019 Shen, Qu, Harata-Lee, Aung, Cui,Wang, Kortschak and Adelson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
DOI: 10.3389/fonc.2019.00632
Appears in Collections:Aurora harvest 4
Molecular and Biomedical Science publications

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