Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/12972
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Type: Journal article
Title: Multiple glucosyltransferase activities in the grapevine Vitis vinifera L
Author: Ford, C.
Hoj, P.
Citation: Australian Journal of Grape and Wine Research, 1998; 4(2):48-58
Publisher: Wiley Interscience
Issue Date: 1998
ISSN: 1322-7130
1755-0238
Abstract: The conjugation by glycosyltransferases of sugars to primary and secondary metabolites is widespread among plants and almost certainly a prerequisite for the accumulation of secondary metabolites at high levels. In the case of the grapevine, Vitis vinifera, modulating the levels of specific secondary berry metabolites is a desirable outcome for the development of wines with particular style characteristics. This can be achieved only by a thorough understanding of the processes underlying glucoside formation and accumulation during berry development. Using protein extracts prepared from leaves and berries of Vitis vinifera cvs, we show here that glucosyltransferase activities can be detected against a wide range of substrates. Among the substrates glucosylated were several classes of phenylpropanoids, including flavonols, anthocyanidins, flavanones, flavones, isoflavones, and a stilbene. Additionally, simple phenols and monoterpenes were glucosylated. Total soluble leaf proteins subjected to ion-exchange chromatography separated into fractions with differing glucosyltransferase activities. This provided strong evidence for the existence both of multiple distinct enzyme activities, and multiple isozymes catalysing identical reactions. Polyclonal antiserum raised to a V. vinifera UDP-glucose:anthocyanidin glucosyltransferase was used to demonstrate the existence of multiple glucosyltransferases in berries and leaves of the grapevine cvs Muscat of Alexandria and Shiraz, thereby confirming data obtained previously through biochemical analyses of recombinant glucosyltransferase.
Keywords: glucosyltransferase; glucosylation; secondary metabolism; Vitis vinifera; flavonoids
Description: © 2008 Australian Society of Viticulture and Oenology Published online at www.interscience.wiley.com
RMID: 0030003823
DOI: 10.1111/j.1755-0238.1998.tb00134
Appears in Collections:Wine Science publications

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