Sequences of the genomic DNAs encoding the S2, S9, S10, and S23 alleles from almond, Prunus dulcis

Abstract

Partial genomic sequences of the S-alleles S2, S9, and S10, identified in genomic DNA selected from Prunus dulcis ´Cristomorto` (S1S2), ´Anxaneta` (S2S9), ´Primorskiy` (S5S9), and ´Gabaix` (S5S10), were found to contain introns of 562, 1,343, and ∼705 bp, and partial exons of 537, 486, and 495 bp respectively. In addition, another allele, characterized as S23, was identified in the two Australian cultivars Johnston`s Prolific and Pierce. This allele had an intron size of 662 bp and a partial exon size of 489 bp, and may have been introduced into Australia in the Spanish cultivar Ramillete (S6S23). The exoni/ntron splice junction sites of all alleles followed the GT/AG consensus sequence rule, and the sequences were found to be highly conserved.