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https://hdl.handle.net/2440/13093
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Type: | Journal article |
Title: | Regeneration of almond from immature seed cotyledons |
Author: | Ainsley, P. Hammerschlag, F. Bertozzi, T. Collins, G. Sedgley, M. |
Citation: | Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants, 2001; 67(3):221-226 |
Publisher: | Kluwer Academic Publ |
Issue Date: | 2001 |
ISSN: | 0167-6857 |
Statement of Responsibility: | Phillip J. Ainsley, Freddi A. Hammerschlag, Terry Bertozzi, Graham G. Collins and Margaret Sedgley |
Abstract: | Adventitious shoots were regenerated from immature cotyledons of four almond cultivars (`Ne Plus Ultra', `Nonpareil', `Carmel' and `Parkinson'). Open-pollinated fruit were collected from orchard-grown trees 100–115 days after full bloom. The proximal ends of the cotyledons were excised and the embryonic axes discarded. The effects of different concentrations of thidiazuron (TDZ) and indole-3-butyric acid (IBA) and the presence or absence of light for the first 7 days of culture were determined. Shoot regeneration rates were highest for cotyledons cultured for 8 weeks on Murashige and Skoog (MS) basal medium containing TDZ (10.0 mgrM), followed by 4 weeks on medium without plant growth regulators. Regeneration levels were further improved if cotyledons were maintained in darkness for the first 7 days. IBA (0.5 mgrM) significantly reduced the development of adventitious shoots. The frequency of cotyledons that developed adventitious shoots under the optimum tested conditions for `Ne Plus Ultra', `Nonpareil', `Carmel', and `Parkinson' were 80.0%, 73.3%, 100.0% and 86.7%, respectively. |
Keywords: | adventitious shoots indole-3-butyric acid organogenesis Prunus dulcis Mill thidiazuron |
Description: | The original publication can be found at www.springerlink.com |
Rights: | © 2001 Kluwer Academic Publishers |
DOI: | 10.1023/A:1012700714085 |
Published version: | http://dx.doi.org/10.1023/a:1012700714085 |
Appears in Collections: | Aurora harvest 2 Environment Institute publications Wine Science publications |
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