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Type: Journal article
Title: Class-switch recombination occurs infrequently in germinal centers
Author: Roco, J.A.
Mesin, L.
Binder, S.C.
Nefzger, C.
Gonzalez-Figueroa, P.
Canete, P.F.
Ellyard, J.
Shen, Q.
Robert, P.A.
Cappello, J.
Vohra, H.
Zhang, Y.
Nowosad, C.R.
Schiepers, A.
Corcoran, L.M.
Toellner, K.M.
Polo, J.M.
Meyer-Hermann, M.
Victora, G.D.
Vinuesa, C.G.
Citation: Immunity, 2019; 51(2):337-350.e7
Publisher: Elsevier
Issue Date: 2019
ISSN: 1074-7613
Statement of
Jonathan A.Roco, Luka Mesin, Sebastian C.Binder, Christian Nefzger, Paula Gonzalez-Figueroa, Pablo F.Canete ... et al.
Abstract: Class-switch recombination (CSR) is a DNA recombination process that replaces the immunoglobulin (Ig) constant region for the isotype that can best protect against the pathogen. Dysregulation of CSR can cause self-reactive BCRs and B cell lymphomas; understanding the timing and location of CSR is therefore important. Although CSR commences upon T cell priming, it is generally considered a hallmark of germinal centers (GCs). Here, we have used multiple approaches to show that CSR is triggered prior to differentiation into GC B cells or plasmablasts and is greatly diminished in GCs. Despite finding a small percentage of GC B cells expressing germline transcripts, phylogenetic trees of GC BCRs from secondary lymphoid organs revealed that the vast majority of CSR events occurred prior to the onset of somatic hypermutation. As such, we have demonstrated the existence of IgM-dominated GCs, which are unlikely to occur under the assumption of ongoing switching.
Keywords: Mice, Inbred C57BL
Rights: © 2019 Elsevier Inc.
DOI: 10.1016/j.immuni.2019.07.001
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Appears in Collections:Medical Sciences publications

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