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https://hdl.handle.net/2440/133479
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Type: | Journal article |
Title: | An improved reprogrammable mouse model harbouring the reverse tetracycline-controlled transcriptional transactivator 3 |
Author: | Alaei, S. Knaupp, A.S. Lim, S.M. Chen, J. Holmes, M.L. Änkö, M.L. Nefzger, C.M. Polo, J.M. |
Citation: | Stem Cell Research, 2016; 17(1):49-53 |
Publisher: | Elsevier |
Issue Date: | 2016 |
ISSN: | 1873-5061 1876-7753 |
Statement of Responsibility: | S. Alaei, A.S. Knaupp, S.M.Lim, J.Chen, M.L.Holmes, M.L.Änköa |
Abstract: | Reprogrammable mouse models engineered to conditionally express Oct-4, Klf-4, Sox-2 and c-Myc (OKSM) have been instrumental in dissecting molecular events underpinning the generation of induced pluripotent stem cells. However, until now these models have been reported in the context of the m2 reverse tetracycline-controlled transactivator, which results in low reprogramming efficiency and consequently limits the number of reprogramming intermediates that can be isolated for downstream profiling. Here, we describe an improved OKSM mouse model in the context of the reverse tetracycline-controlled transactivator 3 with enhanced reprogramming efficiency (>9-fold) and increased numbers of reprogramming intermediate cells albeit with similar kinetics, which we believe will facilitate mechanistic studies of the reprogramming process. |
Keywords: | Cells, Cultured Fibroblasts Animals Mice Teratoma Tetracyclines Proto-Oncogene Proteins c-myc Cell Differentiation Plasmids Octamer Transcription Factor-3 Kruppel-Like Transcription Factors Transcriptional Activation SOXB1 Transcription Factors Induced Pluripotent Stem Cells Cellular Reprogramming |
Rights: | © 2016 The Author(s). Published by Elsevier B.V. |
DOI: | 10.1016/j.scr.2016.05.008 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1092280 http://purl.org/au-research/grants/nhmrc/1085302 |
Published version: | http://dx.doi.org/10.1016/j.scr.2016.05.008 |
Appears in Collections: | Medical Sciences publications |
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