Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/139787
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Apicobasal RNA asymmetries regulate cell fate in the early mouse embryo |
Author: | Hawdon, A. Geoghegan, N.D. Mohenska, M. Elsenhans, A. Ferguson, C. Polo, J.M. Parton, R.G. Zenker, J. |
Citation: | Nature Communications, 2023; 14(1):1-18 |
Publisher: | Nature Research (part of Springer Nature) |
Issue Date: | 2023 |
ISSN: | 2041-1723 2041-1723 |
Statement of Responsibility: | Azelle Hawdon, Niall D. Geoghegan, Monika Mohenska, Anja Elsenhans, Charles Ferguson, Jose M. Polo, Robert G. Parton, Jennifer Zenker |
Abstract: | The spatial sorting of RNA transcripts is fundamental for the refinement of gene expression to distinct subcellular regions. Although, in non-mammalian early embryogenesis, differential RNA localisation presages cell fate determination, in mammals it remains unclear. Here, we uncover apical-to-basal RNA asymmetries in outer blastomeres of 16-cell stage mouse preimplantation embryos. Basally directed RNA transport is facilitated in a microtubule- and lysosome-mediated manner. Yet, despite an increased accumulation of RNA transcripts in basal regions, higher translation activity occurs at the more dispersed apical RNA foci, demonstrated by spatial heterogeneities in RNA subtypes, RNA-organelle interactions and translation events. During the transition to the 32-cell stage, the biased inheritance of RNA transcripts, coupled with differential translation capacity, regulates cell fate allocation of trophectoderm and cells destined to form the pluripotent inner cell mass. Our study identifies a paradigm for the spatiotemporal regulation of post-transcriptional gene expression governing mammalian preimplantation embryogenesis and cell fate. |
Keywords: | Cell lineage; embryonic stem cells; lysosomes; microtubules; ribosome |
Rights: | © The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. |
DOI: | 10.1038/s41467-023-38436-2 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/2002507 http://purl.org/au-research/grants/nhmrc/2009409 http://purl.org/au-research/grants/nhmrc/2004627 http://purl.org/au-research/grants/nhmrc/1140064 http://purl.org/au-research/grants/nhmrc/1150083 http://purl.org/au-research/grants/nhmrc/1156489 |
Published version: | http://dx.doi.org/10.1038/s41467-023-38436-2 |
Appears in Collections: | Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_139787.pdf | Published version | 32.8 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.