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https://hdl.handle.net/2440/14460
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dc.contributor.author | Sallustio, B. | - |
dc.contributor.author | Fairchild, B. | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Journal of Chromatography A, 1995; 665(2):345-353 | - |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.issn | 1879-1794 | - |
dc.identifier.uri | http://hdl.handle.net/2440/14460 | - |
dc.description.abstract | Gemfibrozil 1-O-β-acylglucuronide was purified from the urine of a volunteer administered gemfibrozil, and an isocratic reversed-phase HPLC method was developed for its direct measurement. Quantitation of gemfibrozil and gemfibrozil 1-O-β-acylglucuronide was carried out from plasma, following extraction from acidified specimens into ethyl acetate, on a 5-μm CN reversed-phase column with a mobile phase (pH 3.5) containing acetonitrile, tetrabutylammonium sulphate and distilled water, using fluorescence detection at 284 nm excitation and 316 nm emission. Calibration curves were linear for both compounds over a concentration range of 0.1 to 40 mg/l, with intra-assay coefficients of variation <5% at concentrations of 20.0, 2.0 and 0.2 mg/l, and inter-assay coefficients of variation <10%. No degradation of gemfibrozil 1-O-β-acylglucuronide was detected as a result of the analytical procedure. However, a preliminary application of the method indicates that gemfibrozil acylglucuronide is chemically unstable undergoing intra-molecular rearrangement and hydrolysis under physiological conditions. | - |
dc.description.statementofresponsibility | Benedetta C. Sallustio, Barbara A. Fairchild | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.rights | Copyright © 1995 Published by Elsevier B.V. | - |
dc.source.uri | http://dx.doi.org/10.1016/0378-4347(94)00530-i | - |
dc.subject | Humans | - |
dc.subject | Gemfibrozil | - |
dc.subject | Glucuronates | - |
dc.subject | Chromatography, High Pressure Liquid | - |
dc.subject | Chromatography, Thin Layer | - |
dc.subject | Magnetic Resonance Spectroscopy | - |
dc.subject | Drug Stability | - |
dc.subject | Hydrogen-Ion Concentration | - |
dc.subject | Quality Control | - |
dc.subject | Mass Spectrometry | - |
dc.title | Biosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-β-acylglucuronide | - |
dc.title.alternative | Biosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-beta-acylglucuronide | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/0378-4347(94)00530-I | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Sallustio, B. [0000-0002-0186-3073] | - |
Appears in Collections: | Aurora harvest 2 Pharmacology publications |
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