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Type: Journal article
Title: The response to oxidative stress of Fusobacterium nucleatum grown in continuous culture
Author: Diaz, P.
Zilm, P.
Rogers, A.
Citation: FEMS Microbiology Letters, 2000; 187(1):31-34
Publisher: Elsevier Science BV
Issue Date: 2000
ISSN: 0378-1097
Abstract: Fusobacterium nucleatum ATCC 10953 was grown in continuous culture and the atmosphere changed stepwise from nitrogen (anaerobiosis) to a mixture of air: oxygen (40:60). No significant differences in biomass were observed and the baseline low level of superoxide dismutase increased only slightly; catalase and peroxidase activities were never detected but the level of NADH oxidase increased more than three-fold when oxygen was introduced into the system. In relation to acidic end-products, the relative proportion of acetate increased while that of butyrate decreased. Due mainly, it would seem, to NADH oxidase activity, the culture maintained a low redox potential (Eh=−274 mV) even under an atmosphere of 40% oxygen in air and dissolved oxygen was not detected. This may, in part, explain the protective role of F. nucleatum for anaerobes in both biofilm and planktonic phases of aerated, mixed cultures of oral bacteria.
Keywords: Oxidative stress; Continuous culture; Redox potential; NADH oxidase; Superoxide dismutase; Fusobacterium nucleatum
RMID: 0001000102
DOI: 10.1016/S0378-1097(00)00174-9
Appears in Collections:Dentistry publications

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