Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/16700
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Type: Journal article
Title: Aspirin induces apoptosis in oesophageal cancer cells by inhibiting the pathway of NF-kappaB downstream regulation of cyclooxygenase-2
Author: Liu, J.
Jamieson, G.
Drew, P.
Zhu, G.
Zhang, S.
Zhu, T.
Shan, B.
Wang, Q.
Citation: ANZ Journal of Surgery, 2005; 75(11):1011-1016
Publisher: Blackwell Science Asia
Issue Date: 2005
ISSN: 1445-1433
1445-2197
Statement of
Responsibility: 
Jun-Feng Liu, Glyn G. Jamieson, Paul A. Drew, Gui-Jun Zhu, Shao-Wei Zhang, Tie-Nian Zhu, Bao-En Shan, Qi-Zhang Wang
Abstract: <h4>Background</h4>Aspirin has potential in the prevention or treatment of oesophageal cancer, the seventh most common cancer in the world, but its mechanism of action is still not certain.<h4>Methods</h4>The oesophageal squamous cell carcinoma cell line TE-13 was cultured with aspirin at different concentrations or for different times. Proliferation and apoptosis were measured by MTT reduction and flow cytometry. Expression of COX-2 mRNA was measured by RT-PCR and COX-2 protein levels with Western blot analysis. Nuclear NF-kappaB and cytoplasmic IkappaB protein levels were determined by electrophoretic mobility shift assay and Western blot, respectively.<h4>Results</h4>Aspirin significantly inhibited cell proliferation and induced apoptosis at concentrations of 1, 4, 8 mmol/L. Aspirin dose-dependently decreased the levels of COX-2 mRNA, COX-2 protein and nuclear NF-kappaB protein and increased the cytoplasmic IkappaB protein.<h4>Conclusion</h4>We conclude that aspirin inhibits the proliferation of, and induced apoptosis in, the cultured TE-13 SCC cell line. These changes correlate with a reduction in COX-2 mRNA and protein expression, prostaglandin synthesis, an inhibition of NF-kappaB nuclear translocation, and an increase in cytoplasmic IkappaB. These results support the further investigation of the cyclooxygenase pathway in investigating the potential of aspirin and similar drugs in cancer prevention and therapy.
Keywords: Cell Line, Tumor
Cell Nucleus
Cytoplasm
Humans
Carcinoma, Squamous Cell
Esophageal Neoplasms
Aspirin
Prostaglandins
NF-kappa B
Membrane Proteins
RNA, Messenger
Cell Division
Apoptosis
Cyclooxygenase 2
DOI: 10.1111/j.1445-2197.2005.03596.x
Appears in Collections:Aurora harvest 2
Surgery publications

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