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Type: Journal article
Title: The α-globin gene family of an Australian marsupial, Macropus eugenii: The long evolutionary history of the θ-globin gene and its functional status in mammals
Other Titles: The alpha-globin gene family of an Australian marsupial, Macropus eugenii: The long evolutionary history of the theta-globin gene and its functional status in mammals
Author: Cooper, S.
Wheeler, D.
Hope, R.
Dolman, G.
Saint, K.
Gooley, A.
Holland, R.
Citation: Journal of Molecular Evolution, 2005; 60(5):653-664
Publisher: Springer
Issue Date: 2005
ISSN: 0022-2844
Statement of
Steven J.B. Cooper, David Wheeler, Rory M. Hope, Gaynor Dolman, Kathleen M. Saint, Andrew A. Gooley and Robert A.B. Holland
Abstract: Comparative evolutionary analyses of gene families among divergent lineages can provide information on the order and timing of major gene duplication events and evolution of gene function. Here we investigate the evolutionary history of the α-globin gene family in mammals by isolating and characterizing α-like globin genes from an Australian marsupial, the tammar wallaby, Macropus eugenii. Sequence and phylogenetic analyses indicate that the tammar α -globin family consists of at least four genes including a single adult-expressed gene (α), two embryonic/neonatally expressed genes (ζ and ζ′), and θ -globin, each orthologous to the respective α-, ζ-, and θ-globin genes of eutherian mammals. The results suggest that the θ -globin lineage arose by duplication of an ancestral adult α-globin gene and had already evolved an unusual promoter region, atypical of all known α-globin gene promoters, prior to the divergence of the marsupial and eutherian lineages. Evolutionary analyses, using a maximum likelihood approach, indicate that θ -globin, has evolved under strong selective constraints in both marsupials and the lineage leading to human θ -globin, suggesting a long-term functional status. Overall, our results indicate that at least a four-gene cluster consisting of three α-like and one β-like globin genes linked in the order 5′–ζ–α–θ–ω–3′ existed in the common ancestor of marsupials and eutherians. However, results are inconclusive as to whether the two tammar ζ-globin genes arose by duplication prior to the radiation of the marsupial and eutherian lineages, with maintenance of exon sequences by gene conversion, or more recently within marsupials.
Keywords: Animals
DNA Primers
Likelihood Functions
Sequence Analysis, DNA
Evolution, Molecular
Amino Acid Sequence
Base Sequence
Multigene Family
Models, Genetic
Molecular Sequence Data
Promoter Regions, Genetic
Selection, Genetic
Description: Copyright Springer Science & Business Media B.V.
DOI: 10.1007/s00239-004-0247-9
Appears in Collections:Aurora harvest 6
Environment Institute Leaders publications
Environment Institute publications
Molecular and Biomedical Science publications

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