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Type: Journal article
Title: A family of RS domain proteins with novel subcellular localization and trafficking
Author: Kavanagh, S.
Schulz, T.
Davey, P.
Claudianos, C.
Russell, C.
Rathjen, P.
Citation: Nucleic Acids Research, 2005; 33(4):1309-1322
Publisher: Oxford Univ Press
Issue Date: 2005
ISSN: 0305-1048
Organisation: Centre for the Molecular Genetics of Development
Abstract: We report the sequence, conservation and cell biology of a novel protein, Psc1, which is expressed and regulated within the embryonic pluripotent cell population of the mouse. The Psc1 sequence includes an RS domain and an RNA recognition motif (RRM), and a sequential arrangement of protein motifs that has not been demonstrated for other RS domain proteins. This arrangement was conserved in a second mouse protein (BAC34721. The identification of Psc1 and BAC34721homologues in vertebrates and related proteins, more widely throughout evolution, defines a new family of RS domain proteins termed acidic rich RS (ARRS) domain proteins. Psc1 incorporated into the nuclear speckles, but demonstrated novel aspects of subcellular distribution including localization to speckles proximal to the nuclear periphery and localization to punctate structures in the cytoplasm termed cytospeckles. Integration of Psc1 into cytospeckles was dependent on the RRM. Cytospeckles were dynamic within the cytoplasm and appeared to traffic into the nucleus. These observations suggest a novel role in RNA metabolism for ARRS proteins.
Keywords: COS Cells
Cell Nucleus
Cell Nucleus Structures
Cytoplasmic Structures
RNA-Binding Proteins
Nuclear Proteins
DNA, Complementary
Evolution, Molecular
Binding Sites
Amino Acid Sequence
Conserved Sequence
Protein Structure, Tertiary
Active Transport, Cell Nucleus
Molecular Sequence Data
Chlorocebus aethiops
DOI: 10.1093/nar/gki269
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Centre for the Molecular Genetics of Development publications
Molecular and Biomedical Science publications

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