Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/27982
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | The antibiotic and anticancer active aurein peptides from the Australian Bell Frogs Litoria aurea and Litoria raniformis. Part 2. Sequence determination using electrospray mass spectrometry |
Author: | Rozek, T. Bowie, J. Wallace, J. Tyler, M. |
Citation: | Rapid Communications in Mass Spectrometry, 2000; 14(21):2002-2011 |
Publisher: | John Wiley & Sons Ltd |
Issue Date: | 2000 |
ISSN: | 0951-4198 1097-0231 |
Statement of Responsibility: | Tomas Rozek, John H. Bowie, John C. Wallace and Michael J. Tyler |
Abstract: | Sixteen aurein peptides are present in the host defence secretion from the granular dorsal glands of the Green and Golden Bell Frog Litoria aureus and seventeen from those of the related Southern Bell Frog Litoria raniformis. All peptides have been sequenced using a combination of electrospray mass spectrometry and Lys-C digestion, with each sequence confirmed by automated Edman sequencing. The peptides are named in five groups, viz. aureins 1-5. Ten of these peptides are common to both species of frog. Thirteen of the aurein peptides show wide-spectrum antibiotic and anticancer activity. Amongst the more active peptides are aurein 1.2 (GLFDIIKKIAESF-NH2), the smallest peptide from an anuran reported to have both antibiotic and anticancer activity; aurein 2.2 (GLLDIVKKVIGAFGSL-NH2) and aurein 3.1 (GLFDIVKKIAGHIAGSI-NH2). The aurein 4 and 5 peptides, e.g. aurein 4.1 (GLIQTIKEKLKELAGGLVTGIQS-OH) and aurein 5.1 (GLLDIVTGLLGNLIVDVLKPKTPAS-OH), show neither antibacterial nor anticancer activity. |
Keywords: | Animals Anura Metalloendopeptidases Peptides Antineoplastic Agents Anti-Bacterial Agents Nuclear Magnetic Resonance, Biomolecular Spectrometry, Mass, Electrospray Ionization Sequence Analysis, Protein Amino Acid Sequence Protein Conformation Models, Molecular Molecular Sequence Data |
Description: | Published in Rapid Communications in Mass Spectrometry, 2000; 14 (21):2002-2011 at www.interscience.wiley.com |
DOI: | 10.1002/1097-0231(20001115)14:21<2002::AID-RCM128>3.0.CO;2-3 |
Published version: | http://www3.interscience.wiley.com/cgi-bin/fulltext/73503687/PDFSTART |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.