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https://hdl.handle.net/2440/28044
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dc.contributor.author | Jitrapakdee, S. | - |
dc.contributor.author | Petchamphai, N. | - |
dc.contributor.author | Sunyakumthorn, P. | - |
dc.contributor.author | Wallace, J. | - |
dc.contributor.author | Boonsaeng, V. | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, 2001; 287(2):411-417 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/2440/28044 | - |
dc.description.abstract | We have cloned and sequenced the gene encoding mouse pyruvate carboxylase (mPC) [EC 6.4.1.1]. The coding region contains 19 exons, one 5′-untranslated region exon, and 19 introns in 22 kb of genomic DNA. This gene's exon/intron organization is highly conserved with respect to rat and human PC genes. The mPC gene promoter lacks canonical TATA and CCAAT boxes, in common with a number of housekeeping genes. Transient expressions in COS-1 of a luciferase reporter gene under the control of 5′-nested deletions of the 5′-flanking sequence of the mPC gene have identified the 166-bp minimal sequence required for basal transcription. Alternative splicing at the 5′-untranslated region exon of the mouse PC gene results in the production of two alternate transcripts bearing different 5′-noncoding regions. Both transcripts are highly expressed in kidney and liver and moderately expressed in heart and testis and expressed at a low level in spleen. | - |
dc.description.statementofresponsibility | Sarawut Jitrapakdee; Nathida Petchamphai; Piyanate Sunyakumthorn; John C. Wallace; Vichai Boonsaeng | - |
dc.language.iso | en | - |
dc.publisher | Academic Press Inc | - |
dc.rights | © 2001 Academic Press. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1006/bbrc.2001.5599 | - |
dc.subject | pyruvate carboxylase | - |
dc.subject | biotin-dependent enzyme | - |
dc.subject | gluconeogenesis | - |
dc.subject | lipogenesis | - |
dc.subject | gene regulation | - |
dc.subject | gene structure | - |
dc.title | Structural and promoter regions of the murine pyruvate carboxylase gene | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1006/bbrc.2001.5599 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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