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https://hdl.handle.net/2440/28076
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Carrick, F. | - |
dc.contributor.author | Forbes, B. | - |
dc.contributor.author | Wallace, J. | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | Journal of Biological Chemistry, 2001; 276(29):27120-27128 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2440/28076 | - |
dc.description | Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.description.abstract | In the absence of a complete tertiary structure to define the molecular basis of the high affinity binding interaction between insulin-like growth factors (IGFs) and IGF-binding proteins (IGFBPs), we have investigated binding of IGFs by discrete amino-terminal domains (amino acid residues 1-93, 1-104, 1-132, and 1-185) and carboxyl-terminal domains (amino acid residues 96-279, 136-279, and 182-284) of bovine IGFBP-2 (bIGFBP-2). Both halves of bIGFBP-2 bound IGF-I and IGF-II in BIAcore studies, albeit with different affinities (1-132IGFBP-2, KD = 36.3 and 51.8 nM; 136-279IGFBP-2HIS, KD = 23.8 and 16.3 nM, respectively). The amino-terminal half appears to contain components responsible for fast association. In contrast, IGF binding by the carboxyl-terminal fragment results in a more stable complex as reflected by its KD. Furthermore, des(1-3)IGF-I and des(1-6)IGF-II exhibited reduced binding affinity to 1-279IGFBP-2HIS, 1-132IGFBP-2, and 136-279IGFBP-2HIS biosensor surfaces compared with wild-type IGF. A charge reversal at positions 3 and 6 of IGF-I and IGF-II, respectively, affects binding interactions with the amino-terminal fragment and full-length bIGFBP-2 but not the carboxyl-terminal fragment. | - |
dc.description.statementofresponsibility | Francine E. Carrick, Briony E. Forbes, and John C. Wallace | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Biochemistry Molecular Biology Inc | - |
dc.source.uri | http://dx.doi.org/10.1074/jbc.m101317200 | - |
dc.title | BIAcore analysis of bovine insulin-like growth factor (IGF)-binding protein-2 identifies major IGF binding site determinants in both the amino- and carboxyl-terminal domains | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1074/jbc.M101317200 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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