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https://hdl.handle.net/2440/28209
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Type: | Journal article |
Title: | The classical pathway is the dominant complement pathway required for innate immunity to Streptococcus pneumoniae infection in mice |
Author: | Brown, J. Hussell, T. Gilliland, S. Holden, D. Paton, J. Ehrenstein, M. Walport, M. Botto, M. |
Citation: | Proceedings of the National Academy of Sciences of USA, 2002; 99(26):16969-16974 |
Publisher: | Natl Acad Sciences |
Issue Date: | 2002 |
ISSN: | 0027-8424 1091-6490 |
Statement of Responsibility: | Jeremy S. Brown, Tracy Hussell, Sarah M. Gilliland, David W. Holden, James C. Paton, Michael R. Ehrenstein, Mark J. Walport, and Marina Botto |
Abstract: | The complement system is an important component of the innate immune response to bacterial pathogens, including Streptococcus pneumoniae. The classical complement pathway is activated by antibody–antigen complexes on the bacterial surface and has been considered predominately to be an effector of the adaptive immune response, whereas the alternative and mannose-binding lectin pathways are activated directly by bacterial cell surface components and are considered effectors of the innate immune response. Recently, a role has been suggested for the classical pathway during innate immunity that is activated by natural IgM or components of the acute-phase response bound to bacterial pathogens. However, the functional importance of the classical pathway for innate immunity to S. pneumoniae and other bacterial pathogens, and its relative contribution compared with the alternative and mannose-binding lectin pathways has not been defined. By using strains of mice with genetic deficiencies of complement components and secretory IgM we have investigated the role of each complement pathway and natural IgM for innate immunity to S. pneumoniae. Our results show that the proportion of a population of S. pneumoniae bound by C3 depends mainly on the classical pathway, whereas the intensity of C3 binding depends on the alternative pathway. Furthermore, the classical pathway, partially targeted by the binding of natural IgM to bacteria, is the dominant pathway for activation of the complement system during innate immunity to S. pneumoniae, loss of which results in rapidly progressing septicemia and impaired macrophage activation. These data demonstrate the vital role of the classical pathway for innate immunity to a bacterial pathogen. |
Keywords: | Animals Mice, Inbred C57BL Mice Streptococcus pneumoniae Pneumococcal Infections Immunoglobulin G Immunoglobulin M Complement Pathway, Alternative Complement Pathway, Classical Complement C3 Complement C1q Immunity, Innate |
Rights: | © 2002 by the National Academy of Sciences |
DOI: | 10.1073/pnas.012669199 |
Published version: | http://www.pnas.org/cgi/content/abstract/99/26/16969 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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