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Type: Journal article
Title: Identification of a biological activity that supports maintenance and proliferation of pluripotent cells from the primitive ectoderm of the mouse
Author: Rathjen, J.
Washington, J.
Bettess, M.
Rathjen, P.
Citation: Biology of Reproduction, 2003; 69(6):1863-1871
Publisher: Soc Study Reproduction
Issue Date: 2003
ISSN: 0006-3363
Organisation: Centre for the Molecular Genetics of Development
Statement of
Joy Rathjen, Jennifer M. Washington, Michael D. Bettess and Peter D. Rathjen
Abstract: Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2 cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an alternative technology for the isolation of pluripotent cells from the mammalian embryo.
Keywords: Cells, Cultured
Extracellular Matrix
Pluripotent Stem Cells
Mice, Inbred CBA
Carcinoma, Hepatocellular
Biological Factors
Culture Media, Conditioned
Cell Division
Leukemia Inhibitory Factor
Description: Copyright © 2003 by the Society for the Study of Reproduction, Inc.
Provenance: BOR - Papers in Press, published online ahead of print August 6, 2003.
DOI: 10.1095/biolreprod.103.017384
Appears in Collections:Aurora harvest 2
Centre for the Molecular Genetics of Development publications
Molecular and Biomedical Science publications

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