Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/30876
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorZannettino, A.-
dc.contributor.authorFarrugia, A.-
dc.contributor.authorTo, L.-
dc.contributor.authorAtkins, G.-
dc.contributor.editorBrown, R.-
dc.contributor.editorJoy Ho, P.-
dc.date.issued2005-
dc.identifier.citationMultiple Myeloma: Methods and Protocols, 2005 / Brown, R., Joy Ho, P. (ed./s), vol.113, pp.245-256-
dc.identifier.isbn1592599168-
dc.identifier.urihttp://hdl.handle.net/2440/30876-
dc.description.abstractMuch of the morbidity and mortality associated with the plasma cell (PC) malignancy, multiple myeloma (MM), is owing to the severe osteolytic bone disease seen in patients with this disease. Although the molecular mechanisms responsible for osteolysis remain to be fully elucidated, it is clear from numerous studies that it is owing, in part, to an increase in osteoclastic bone resorption. Several known osteoclast (OC)-activating factors (OAFs) are produced by myeloma PCs (MPCs), or by stromal cells in response to MPCs and include interleukin-1beta (IL-1beta); tumor necrosis factor-alpha (TNF-alpha); IL-6; parathyroid hormone-related protein; macrophage inflammatory protein-1alpha; and, most recently, the TNF-ligand family member receptor activator of nuclear factor-kappaB ligand (RANKL). The identification and significance of any one of these myeloma-derived OAFs is dependent on robust and reliable assays that measure the de novo formation and activation of OCs. A number of in vitro assay systems have been described that examine the requirements for normal OC formation and are easily adaptable for examining which MM-derived OAF and to what extent it is responsible for the bone loss observed in individuals with myeloma. This chapter describes one such in vitro model system.-
dc.language.isoen-
dc.publisherHumana Press-
dc.source.urihttp://dx.doi.org/10.1385/1-59259-916-8:245-
dc.subjectBone and Bones-
dc.subjectPlasma Cells-
dc.subjectLeukocytes, Mononuclear-
dc.subjectOsteoclasts-
dc.subjectHumans-
dc.subjectMultiple Myeloma-
dc.subjectBone Diseases-
dc.subjectBone Resorption-
dc.subjectOsteolysis-
dc.subjectAntigens, CD-
dc.subjectMicroscopy, Electron, Scanning-
dc.subjectCell Culture Techniques-
dc.subjectCoculture Techniques-
dc.subjectLipopolysaccharide Receptors-
dc.titleAn in vitro osteoclast-forming assay to measure myeloma cell-derived osteoclast-activating factors-
dc.typeBook chapter-
dc.identifier.doi10.1385/1-59259-916-8:245-
dc.publisher.placeTotowa, New Jersey-
pubs.publication-statusPublished-
dc.identifier.orcidZannettino, A. [0000-0002-6646-6167]-
dc.identifier.orcidAtkins, G. [0000-0002-3123-9861]-
Appears in Collections:Aurora harvest 6
Orthopaedics and Trauma publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.