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Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/34771
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dc.contributor.authorGrutzner, F.-
dc.contributor.authorTsend-Ayush, E.-
dc.contributor.authorStout, K.-
dc.contributor.authorMunsche, S.-
dc.contributor.authorNiveleau, A.-
dc.contributor.authorNanda, I.-
dc.contributor.authorSchmid, M.-
dc.contributor.authorHaaf, T.-
dc.date.issued2001-
dc.identifier.citationCytogenetic and Genome Research, 2001; 93(3-4):265-269-
dc.identifier.issn1424-8581-
dc.identifier.issn1424-859X-
dc.identifier.urihttp://hdl.handle.net/2440/34771-
dc.descriptionCopyright © 2001 S. Karger AG-
dc.description.abstractMicrodissection of single chicken microchromosomes (MICs) followed by degenerate oligonucleotide-primed (DOP) PCR allows the rapid generation of MIC-specific DNA libraries. Since some libraries derived from a single (or a few) chromosome(s) label the entire MIC fraction, the majority of chicken MICs share repetitive DNA sequences that are not found on the macrochromosomes. In evolutionarily distant bird species, MICs are invariably hypermethylated. Methylcytosine staining provides additional in situ evidence for the high gene content of MICs and strong compartmentalization of avian genomes.-
dc.description.statementofresponsibilityF. Grützner, E. Zend-Ajusch, K. Stout, S. Munsche, A. Niveleau, I. Nanda, M. Schmid and T. Haaf-
dc.language.isoen-
dc.publisherKarger-
dc.subjectChromosomes-
dc.subjectAnimals-
dc.subjectChickens-
dc.subjectPalaeognathae-
dc.subjectDNA Probes-
dc.subjectChromosome Painting-
dc.subjectSensitivity and Specificity-
dc.subjectPolymerase Chain Reaction-
dc.subjectEvolution, Molecular-
dc.subjectDNA Methylation-
dc.subjectGC Rich Sequence-
dc.subjectRepetitive Sequences, Nucleic Acid-
dc.subjectSubstrate Specificity-
dc.subjectGene Library-
dc.subjectGenome-
dc.titleChicken microchromosomes are hypermethylated and can be identified by specific painting probes-
dc.typeJournal article-
dc.identifier.doi10.1159/000056996-
pubs.publication-statusPublished-
dc.identifier.orcidGrutzner, F. [0000-0002-3088-7314]-
Appears in Collections:Aurora harvest
Environment Institute Leaders publications
Molecular and Biomedical Science publications

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