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Type: Journal article
Title: An XX/XY sex microchromosome system in a freshwater turtle, Chelodina longicollis (Testudines: Chelidae) with genetic sex determination
Author: Ezaz, T.
Valenzuela, N.
Grutzner, F.
Miura, I.
Georges, A.
Burke, R.
Graves, J.
Citation: Chromosome Research, 2006; 14(2):139-150
Publisher: Kluwer Academic Publ
Issue Date: 2006
ISSN: 0967-3849
Statement of
Tariq Ezaz , Nicole Valenzuela, Frank Grützner, Ikuo Miura, Arthur Georges, Russell L. Burke and Jennifer A. Marshall Graves
Abstract: Heteromorphic sex chromosomes are rare in turtles, having been described in only four species. Like many turtle species, the Australian freshwater turtle Chelodina longicollis has genetic sex determination, but no distinguishable (heteromorphic) sex chromosomes were identified in a previous karyotyping study. We used comparative genomic hybridization (CGH) to show that C. longicollis has an XX/XY system of chromosomal sex determination, involving a pair of microchromosomes. C-banding and reverse fluorescent staining also distinguished microchromosomes with different banding patterns in males and females in ∼70% cells examined. GTG-banding did not reveal any heteromorphic chromosomes, and no replication asynchrony on the X or Y microchromosomes was observed using replication banding. We conclude that there is a very small sequence difference between X and Y chromosomes in this species, a difference that is consistently detectable only by high-resolution molecular cytogenetic techniques, such as CGH. This is the first time a pair of microchromosomes has been identified as the sex chromosomes in a turtle species.
Keywords: Sex Chromosomes; Animals; Turtles; Chromosome Banding; Staining and Labeling; Karyotyping; Nucleic Acid Hybridization; Sex Differentiation; Female; Male; Sex Determination Processes
RMID: 0020064677
DOI: 10.1007/s10577-006-1029-6
Appears in Collections:Molecular and Biomedical Science publications
Environment Institute Leaders publications

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