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https://hdl.handle.net/2440/39034
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xiao, Y. | - |
dc.contributor.author | Bartold, P. | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Journal of the International Academy of Periodontology, 2004; 6(3):81-88 | - |
dc.identifier.issn | 1466-2094 | - |
dc.identifier.issn | 2518-3745 | - |
dc.identifier.uri | http://hdl.handle.net/2440/39034 | - |
dc.description | Copyright © 2004 Journal of the International Academy of Periodontology | - |
dc.description.abstract | <h4>Objective</h4>Plasminogen activator inhibitor-2 (PAI-2) is an important counter proteolysis factor which helps protect tissues from inflammatory stress. The expression of PAI-2 can be modulated by various inflammatory stimulants and mediators. The aim of the present study was to investigate how serum factors, might modulate the effects of lipopolysaccharide (LPS) and interleukin-1beta on PAI-2 production by human gingival fibroblasts.<h4>Methods</h4>Human gingival fibroblasts were exposed to LPS derived from Actinobacillus actinomycetemcomitans or Escherichia coli and a commercial source of interleukin-1beta (IL-1beta). The expression of PAI-2 and its mRNA was monitored by Western blotting, RT-PCR, and Northern blotting.<h4>Results</h4>The results showed that the distribution of PAI-2 synthesised by human gingival fibroblasts (HGF) was mostly as an intracellular protein (47kDa). The presence of serum in the culture media was absolutely necessary for both the secretion of PAI-2 and for the effect of the inflammatory mediators (LPS and IL-1beta). A pattern of PAI-2 response in HGF after LPS stimulation was detected with a quick up-regulation of the PAI-2 mRNA, which was down regulated when extracellular PAI-2 (60kDa mass) levels reached plateau levels. The synthesis of PAI-2 by HGF, in terms of mRNA expression and protein synthesis, was more sensitive to stimulation with lower concentrations of LPS (10 ng/ml) than with higher LPS concentrations.<h4>Conclusions</h4>PAI-2 is a serum dependent molecule with major cytosolic localisation in HGF. Its cellular accumulation and secretion can be modulated by bacterial LPS and IL-1beta through serum factors. | - |
dc.description.statementofresponsibility | Y. Xiao and P.M. Bartold | - |
dc.language.iso | en | - |
dc.publisher | FDI World Dental Press Ltd. | - |
dc.subject | Cells, Cultured | - |
dc.subject | Fibroblasts | - |
dc.subject | Fetal Blood | - |
dc.subject | Gingiva | - |
dc.subject | Animals | - |
dc.subject | Cattle | - |
dc.subject | Humans | - |
dc.subject | Lipopolysaccharides | - |
dc.subject | Plasminogen Activator Inhibitor 2 | - |
dc.subject | Interleukin-1 | - |
dc.subject | Culture Media, Conditioned | - |
dc.subject | Blotting, Western | - |
dc.subject | Blotting, Northern | - |
dc.subject | Analysis of Variance | - |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject | Gene Expression Regulation | - |
dc.subject | Up-Regulation | - |
dc.subject | Dose-Response Relationship, Drug | - |
dc.title | Modulating effect of serum on the stimulation of plasminogen activator inhibitor 2 production in human gingival fibroblasts by lipopolysaccharide and interleukin-1beta | - |
dc.type | Journal article | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Bartold, P. [0000-0002-5695-3877] | - |
Appears in Collections: | Aurora harvest Dentistry publications |
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