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https://hdl.handle.net/2440/42826
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Type: | Journal article |
Title: | Surfactant protein D expression in chronic rhinosinusitis patients and immune responses in vitro to Aspergillus and Alternaria in a nasal explant model |
Author: | Ooi, E. Wormald, P. Carney, S. James, C. Tan, L. |
Citation: | The Laryngoscope, 2007; 117(1):51-57 |
Publisher: | Lippincott Williams & Wilkins |
Issue Date: | 2007 |
ISSN: | 0023-852X 1531-4995 |
Statement of Responsibility: | Ooi EH, Wormald PJ, Carney AS, James CL, Tan LW. |
Abstract: | Objectives/Hypothesis: Common fungi have been implicated in the pathogenesis of chronic rhinosinusitis (CRS) with eosinophilic mucus (EMCRS). Surfactant protein (SP)-D plays an important role in the immune response to Aspergillus fumigatus in the lungs. We sought to determine whether SP-D is expressed in nasal mucosa and investigated the response of SP-D in vitro to fungal allergens. Study Design and Methods: 1) Nasal biopsies from 59 CRS and EMCRS patients, stratified into allergic fungal sinusitis (AFS), nonallergic fungal eosinophilic sinusitis (NAFES), and nonallergic nonfungal eosinophilic sinusitis (NANFES) were studied by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR), immunostaining and enzyme-linked immunosorbent assay (ELISA). 2) Nasal tissue from three CRS and three NANFES patients was cultured with fungal allergens in a nasal explant in vitro model for 24 hours at increasing concentrations and mRNA SP-D secreted SP-D protein levels in response to the fungi determined by qRT-PCR and ELISA. Results: Staining for SP-D was detected in the submucosal glands from the nasal biopsies in all patient groups except for AFS. By ELISA, SP-D was undetectable in AFS and decreased in NAFES, NANFES, and CRS compared with controls. CRS patients in vitro cultured with Aspergillus and Alternaria allergens in a nasal tissue explant model induced up-regulation of SP-D by qRT-PCR. In contrast, NANFES nasal tissue explants cultured with Aspergillus allergens induced down-regulation of SP-D. Conclusions: We report for the first time the expression of SP-D in both diseased and normal nasal mucosa. SP-D expression in CRS patients is up-regulated by fungal allergens in an in vitro model. These results may provide potential novel therapy for treatment of CRS. |
Keywords: | Nasal Mucosa Mucus Humans Alternaria Aspergillus fumigatus Sinusitis Rhinitis, Allergic, Perennial Eosinophilia Pulmonary Surfactant-Associated Protein D RNA, Messenger Allergens Prospective Studies Reverse Transcriptase Polymerase Chain Reaction Up-Regulation Adult Aged Aged, 80 and over Middle Aged Female Male |
Description: | Copyright ©2007, The Journal of the American Laryngological, Rhinological and Otological Society, Inc. |
DOI: | 10.1097/01.mlg.0000243196.75418.6f |
Description (link): | http://www.ncbi.nlm.nih.gov/pubmed/17202930 |
Published version: | http://dx.doi.org/10.1097/01.mlg.0000243196.75418.6f |
Appears in Collections: | Aurora harvest Surgery publications |
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