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Type: Journal article
Title: Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation
Author: Moghaddami, M.
Cleland, L.
Radisic, G.
Mayrhofer, G.
Citation: Arthritis Research and Therapy, 2007; 9(6):1-13
Publisher: BioMed Central Ltd.
Issue Date: 2007
ISSN: 1478-6354
Statement of
Mahin Moghaddami, Leslie G Cleland, Gorjana Radisic and Graham Mayrhofer
Abstract: Adoptive transfer of adjuvant-induced arthritis was used in this study to examine local macrophages and dendritic cells (DCs) during T cell-mediated synovial inflammation. We studied the influx of CD11b+CD11c+ putative myeloid DCs and other non-lymphoid CD45+ cells into synovium-rich tissues (SRTs) of the affected hind paws in response to a pulse of autoreactive thoracic duct cells. Cells were prepared from the SRTs using a collagenase perfusion-digestion technique, thus allowing enumeration and phenotypic analysis by flow cytometry. Numbers of CD45+ cells increased during the first 6 days, with increases in CD45+MHC (major histocompatibility complex) II+ monocyte-like cells from as early as day 3 after transfer. In contrast, typical MHC II- monocytes, mainly of the CD4- subset, did not increase until 12 to 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells. By day 14, CD45+MHC IIhi cells constituted approximately half of all CD45+ cells in SRT. Most of the MHC IIhi cells expressed CD11c and CD11b and represented putative myeloid DCs, whereas only approximately 20% were CD163+ macrophages. Less than 5% of the MHC IIhi cells in inflamed SRT were CD11b-, setting a maximum for any influx of plasmacytoid DCs. Of the putative myeloid DCs, a third expressed CD4 and both the CD4+ and the CD4- subsets expressed the co-stimulatory molecule CD172a. Early accumulation of MHC IIhiCD11c+ monocyte-like cells during the early phase of T cell-mediated inflammation, relative to typical MHC II- blood monocytes, suggests that recruited monocytes differentiate rapidly toward the DC lineage at this stage in the disease process. However, it is possible also that the MHC IIhiCD11c+ cells originate from a specific subset of DC-like circulating mononuclear cells.
Keywords: Dendritic Cells
Rats, Inbred Strains
Arthritis, Experimental
Intercellular Adhesion Molecule-1
Adoptive Transfer
Cell Movement
CD4 Antigens
Leukocyte Common Antigens
B7-1 Antigen
CD11b Antigen
CD11c Antigen
B7-2 Antigen
Description: This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Rights: Copyright © 2007 Moghaddami et al.; licensee BioMed Central Ltd.
DOI: 10.1186/ar2328
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Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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