Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/43543
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation |
Author: | Moghaddami, M. Cleland, L. Radisic, G. Mayrhofer, G. |
Citation: | Arthritis Research and Therapy, 2007; 9(6):1-13 |
Publisher: | BioMed Central Ltd. |
Issue Date: | 2007 |
ISSN: | 1478-6354 1478-6362 |
Statement of Responsibility: | Mahin Moghaddami, Leslie G Cleland, Gorjana Radisic and Graham Mayrhofer |
Abstract: | Adoptive transfer of adjuvant-induced arthritis was used in this study to examine local macrophages and dendritic cells (DCs) during T cell-mediated synovial inflammation. We studied the influx of CD11b+CD11c+ putative myeloid DCs and other non-lymphoid CD45+ cells into synovium-rich tissues (SRTs) of the affected hind paws in response to a pulse of autoreactive thoracic duct cells. Cells were prepared from the SRTs using a collagenase perfusion-digestion technique, thus allowing enumeration and phenotypic analysis by flow cytometry. Numbers of CD45+ cells increased during the first 6 days, with increases in CD45+MHC (major histocompatibility complex) II+ monocyte-like cells from as early as day 3 after transfer. In contrast, typical MHC II- monocytes, mainly of the CD4- subset, did not increase until 12 to 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells. By day 14, CD45+MHC IIhi cells constituted approximately half of all CD45+ cells in SRT. Most of the MHC IIhi cells expressed CD11c and CD11b and represented putative myeloid DCs, whereas only approximately 20% were CD163+ macrophages. Less than 5% of the MHC IIhi cells in inflamed SRT were CD11b-, setting a maximum for any influx of plasmacytoid DCs. Of the putative myeloid DCs, a third expressed CD4 and both the CD4+ and the CD4- subsets expressed the co-stimulatory molecule CD172a. Early accumulation of MHC IIhiCD11c+ monocyte-like cells during the early phase of T cell-mediated inflammation, relative to typical MHC II- blood monocytes, suggests that recruited monocytes differentiate rapidly toward the DC lineage at this stage in the disease process. However, it is possible also that the MHC IIhiCD11c+ cells originate from a specific subset of DC-like circulating mononuclear cells. |
Keywords: | Dendritic Cells T-Lymphocytes Macrophages Animals Rats, Inbred Strains Rats Arthritis, Experimental Synovitis Intercellular Adhesion Molecule-1 Adoptive Transfer Cell Movement Female CD4 Antigens Leukocyte Common Antigens B7-1 Antigen CD11b Antigen CD11c Antigen B7-2 Antigen |
Description: | This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Rights: | Copyright © 2007 Moghaddami et al.; licensee BioMed Central Ltd. |
DOI: | 10.1186/ar2328 |
Published version: | http://arthritis-research.com/content/9/6/R120 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_43543.pdf | Published version | 1.5 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.