Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/43543
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Type: Journal article
Title: Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation
Author: Moghaddami, M.
Cleland, L.
Radisic, G.
Mayrhofer, G.
Citation: Arthritis Research & Therapy, 2007; 9(6):1-13
Publisher: BioMed Central Ltd.
Issue Date: 2007
ISSN: 1478-6354
1478-6362
Statement of
Responsibility: 
Mahin Moghaddami, Leslie G Cleland, Gorjana Radisic and Graham Mayrhofer
Abstract: Adoptive transfer of adjuvant-induced arthritis was used in this study to examine local macrophages and dendritic cells (DCs) during T cell-mediated synovial inflammation. We studied the influx of CD11b+CD11c+ putative myeloid DCs and other non-lymphoid CD45+ cells into synovium-rich tissues (SRTs) of the affected hind paws in response to a pulse of autoreactive thoracic duct cells. Cells were prepared from the SRTs using a collagenase perfusion-digestion technique, thus allowing enumeration and phenotypic analysis by flow cytometry. Numbers of CD45+ cells increased during the first 6 days, with increases in CD45+MHC (major histocompatibility complex) II+ monocyte-like cells from as early as day 3 after transfer. In contrast, typical MHC II- monocytes, mainly of the CD4- subset, did not increase until 12 to 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells. By day 14, CD45+MHC IIhi cells constituted approximately half of all CD45+ cells in SRT. Most of the MHC IIhi cells expressed CD11c and CD11b and represented putative myeloid DCs, whereas only approximately 20% were CD163+ macrophages. Less than 5% of the MHC IIhi cells in inflamed SRT were CD11b-, setting a maximum for any influx of plasmacytoid DCs. Of the putative myeloid DCs, a third expressed CD4 and both the CD4+ and the CD4- subsets expressed the co-stimulatory molecule CD172a. Early accumulation of MHC IIhiCD11c+ monocyte-like cells during the early phase of T cell-mediated inflammation, relative to typical MHC II- blood monocytes, suggests that recruited monocytes differentiate rapidly toward the DC lineage at this stage in the disease process. However, it is possible also that the MHC IIhiCD11c+ cells originate from a specific subset of DC-like circulating mononuclear cells.
Keywords: Dendritic Cells; T-Lymphocytes; Macrophages; Animals; Rats, Inbred Strains; Rats; Arthritis, Experimental; Synovitis; Intercellular Adhesion Molecule-1; Adoptive Transfer; Cell Movement; Female; CD4 Antigens; Leukocyte Common Antigens; B7-1 Antigen; CD11b Antigen; CD11c Antigen; B7-2 Antigen
Description: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Rights: Copyright © 2007 Moghaddami et al.; licensee BioMed Central Ltd.
RMID: 0020074330
DOI: 10.1186/ar2328
Published version: http://arthritis-research.com/content/9/6/R120
Appears in Collections:Molecular and Biomedical Science publications

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