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Type: Journal article
Title: A pneumococcal MerR-like regulator and S-nitrosoglutathione reductase are required for systemic virulence
Author: Stroeher, U.
Kidd, S.
Stafford, S.
Jennings, M.
Paton, J.
McEwan, A.
Citation: Journal of Infectious Diseases, 2007; 196(12):1820-1826
Publisher: Univ Chicago Press
Issue Date: 2007
ISSN: 0022-1899
Statement of
Uwe H. Stroeher, Robert S. Kidd, Sian L. Stafford, Michael P. Jennings, James C. Paton and Alastair G. McEwan
Abstract: A transcriptional regulator, NmlR(sp), has been identified in Streptococcus pneumoniae that is required for defense against nitric oxide (NO) stress. The nmlR(sp) gene is cotranscribed with adhC, which encodes an alcohol dehydrogenase that is able to reduce S-nitrosoglutathione (GSNO) with NADH as reductant. nmlR(sp) and adhC mutants exhibited a reduced level of NADH-GSNO oxidoreductase activity and were more susceptible to killing by NO than were wild-type cells. Comparison of the virulence of wild-type and mutant strains by use of a mouse model system showed that NmlR(sp) and AdhC do not play a key role in the adherence of pneumococci to the nasopharynx in vivo. An intraperitoneal challenge experiment revealed that both NmlR(sp) and AdhC were required for survival in blood. These data identify novel components of a NO defense system in pneumococci that are required for systemic infection.
Keywords: Nasopharynx; Respiratory Mucosa; Cell Line; Cell Line, Tumor; Animals; Humans; Mice; Neisseria gonorrhoeae; Streptococcus pneumoniae; Nitric Oxide; NAD; Aldehyde Oxidoreductases; Bacterial Proteins; DNA-Binding Proteins; Phylogeny; Virulence; Transcription, Genetic; Base Sequence; Molecular Sequence Data; Female
Description: Copyright © 2007 by the Infectious Diseases Society of America. All rights reserved.
RMID: 0020073997
DOI: 10.1086/523107
Appears in Collections:Molecular and Biomedical Science publications

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