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https://hdl.handle.net/2440/45327
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Type: | Journal article |
Title: | Mast cells, neuropeptides, histamine, and prostaglandins in UV-induced systemic immunosuppression |
Author: | Hart, P. Townley, S. Grimbaldeston, M. Kahlil, Z. Finlay-Jones, J. |
Citation: | Methods, 2002; 28(1):79-89 |
Publisher: | Academic Press Inc Elsevier Science |
Issue Date: | 2002 |
ISSN: | 1046-2023 1095-9130 |
Statement of Responsibility: | Prue H. Hart, Scott L. Townley, Michele A. Grimbaldeston, Zeinab Khalil and John J. Finlay-Jones |
Abstract: | There is a direct correlation between dermal mast cell prevalence in dorsal skin of different mouse strains and susceptibility to UVB-induced systemic immunosuppression; highly UV-susceptible C57BL/6 mice have a high dermal mast cell prevalence while BALB/c mice, which require considerable UV radiation for 50% immunosuppression, have a low mast cell prevalence. There is also a functional link between the prevalence of dermal mast cells and susceptibility to UVB- and cis-urocanic acid (UCA)-induced systemic immunosuppression. Mast cell-depleted mice are unresponsive to UVB or cis-UCA for systemic immunosuppression unless they are previously reconstituted at the irradiated or cis-UCA-administered site with bone marrow-derived mast cell precursors. cis-UCA does not stimulate mast cell degranulation directly. Instead, in support of studies showing that neither UVB nor cis-UCA was immunosuppressive in capsaicin-treated, neuropeptide-depleted mice, cis-UCA-stimulated neuropeptide release from sensory c-fibers which, in turn, could efficiently degranulate mast cells. Studies in mice suggested that histamine, and not tumor necrosis factor alpha (TNF-alpha), was the product from mast cells that stimulated downstream immunosuppression. Histamine receptor antagonists reduced by approximately 60% UVB and cis-UCA-induced systemic immunosuppression. Indomethacin administration to mice had a similar effect which was not cumulative with the histamine receptor antagonists. Histamine can stimulate keratinocyte prostanoid production. We propose that both histamine and prostaglandin E(2) are important in downstream immunosuppression; both are regulatory molecules supporting the development of T helper 2 cells and reduced expression of type 1 immune responses such as a contact hypersensitivity reaction. |
Keywords: | Microcirculation Mast Cells Animals Mice, Inbred Strains Mice Rats Rats, Sprague-Dawley Dermatitis, Contact Histamine Urocanic Acid Indomethacin Prostaglandins Nerve Growth Factor Neuropeptides Ultraviolet Rays Cell Degranulation Immune Tolerance Female Male |
Description: | Copyright © 2002 Elsevier Science B.V. All rights reserved. |
DOI: | 10.1016/S1046-2023(02)00201-3 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/622914/description#description |
Published version: | http://dx.doi.org/10.1016/s1046-2023(02)00201-3 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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