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Type: Journal article
Title: Dose-dependent reduction of dietary protein-induced colonocyte DNA damage by resistant starch in rats correlates more highly with caecal butyrate than with other short chain fatty acids
Author: Toden, S.
Bird, A.
Topping, D.
Conlon, M.
Citation: Cancer Biology and Therapy, 2007; 6(2):253-258
Publisher: Landes Bioscience
Issue Date: 2007
ISSN: 1538-4047
Statement of
Shusuke Toden, Anthony R. Bird, David L. Topping and Michael A. Conlon
Abstract: Previous studies have shown increased levels of colonocyte DNA damage (as measured by the comet assay) and thinning of the colonic mucus layer in rats fed higher dietary protein as casein or red meat with highly digestible starch. Feeding resistant starch (RS) as high amylose maize starch (HAMS) opposed these changes. However, the dietary level of HAMS was relatively high (48% by weight) so this study was conducted to establish whether HAMS had the same effects at lower dietary levels. Adult male rats were fed a diet containing 25% casein with 0%, 10%, 20%, 30% or 40% HAMS for 4 wk. DNA single strand breaks and 8-hydroxyguanosine levels were measured in isolated colonocytes by the comet assay. As expected, comet tail moment was greatest and the mucus barrier thinnest in rats fed 0% HAMS. DNA damage was reduced and the mucus barrier thickened in a logarithmic dose-dependent manner by HAMS. There was no significant difference in 8-hydroxyguanosine between dietary groups. Caecal and fecal short chain fatty acid (SCFA) pools rose with the increased level of dietary HAMS. DNA damage of colonocytes correlated negatively with caecal SCFA but the strongest correlation was with caecal butyrate, which is consistent with the proposed role of this SCFA in promoting a normal cell phenotype. These data show that HAMS prevents protein-induced colonic DNA damage in a dose-dependent manner. Inclusion of 10% HAMS was found to be sufficient to oppose colonocyte DNA damage, and to increase caecal and fecal SCFA pools.
Keywords: Intestinal Mucosa
Intestine, Large
Rats, Sprague-Dawley
Zea mays
Intestinal Diseases
DNA Damage
Dietary Carbohydrates
Fatty Acids, Volatile
Dietary Proteins
Plant Preparations
Dose-Response Relationship, Drug
Edible Grain
Description: Copyright © 2007 Landes Bioscience
DOI: 10.4161/cbt.6.2.3627
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Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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