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https://hdl.handle.net/2440/47173
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dc.contributor.author | Kumar, R. | - |
dc.contributor.author | Cheney, K. | - |
dc.contributor.author | McKirdy, R. | - |
dc.contributor.author | Neilsen, P. | - |
dc.contributor.author | Schulz, R. | - |
dc.contributor.author | Lee, J. | - |
dc.contributor.author | Cohen, J. | - |
dc.contributor.author | Booker, G. | - |
dc.contributor.author | Callen, D. | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Journal of Biological Chemistry, 2008; 283(27):19026-19038 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | http://hdl.handle.net/2440/47173 | - |
dc.description | Copyright © 2008 by the American Society for Biochemistry and Molecular Biology. | - |
dc.description.abstract | Transcriptional repression plays a critical role in development and homeostasis. The ETO family represents a group of highly conserved and ubiquitously expressed transcriptional regulatory proteins that are components of a diverse range of multiprotein repressor complexes. ETO proteins function as transcriptional repressors by interacting with a number of transcription factors that bind to their cognate consensus DNA binding sequences within the promoters of target genes. We previously reported that the classical C2H2 zinc finger DNA-binding protein, ZNF652, specifically and functionally interacts with the ETO protein CBFA2T3 and has a role in the suppression of breast oncogenesis. Here we report the identification and validation of the ZNF652 consensus DNA binding sequence. Our results show that the E-box gene HEB is a direct target of CBFA2T3-ZNF652-mediated transcriptional repression. The CBFA2T3-ZNF652 complex regulates HEB expression by binding to a single ZNF652 response element located within the promoter sequence of HEB. This study also shows that the NHR3 and NHR4 domains of CBFA2T3 interact with a conserved proline-rich region located within the C terminus of ZNF652. Our results, together with previous reports, indicate that HEB has a complex relationship with CBFA2T3; CBFA2T3 interacts with ZNF652 to repress HEB expression, and in addition CBFA2T3 interacts with the HEB protein to inhibit its activator function. These findings suggest that CBFA2T3-ZNF652-mediated HEB regulation may play an important role in hematopoiesis and myogenesis. | - |
dc.description.statementofresponsibility | Raman Kumar, Kelly M. Cheney, Ross McKirdy, Paul M. Neilsen, Renèe B. Schulz, Jaclyn Lee, Juliane Cohen, Grant W. Booker, and David F. Callen | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Biochemistry Molecular Biology Inc | - |
dc.source.uri | http://www.jbc.org/cgi/content/abstract/283/27/19026 | - |
dc.subject | Jurkat Cells | - |
dc.subject | CHO Cells | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Cricetulus | - |
dc.subject | Breast Neoplasms | - |
dc.subject | Cell Transformation, Neoplastic | - |
dc.subject | Multiprotein Complexes | - |
dc.subject | DNA-Binding Proteins | - |
dc.subject | Proto-Oncogene Proteins | - |
dc.subject | Tumor Suppressor Proteins | - |
dc.subject | Phosphoproteins | - |
dc.subject | Transcription Factors | - |
dc.subject | Repressor Proteins | - |
dc.subject | Transcription, Genetic | - |
dc.subject | Gene Expression Regulation, Neoplastic | - |
dc.subject | Response Elements | - |
dc.subject | Protein Structure, Tertiary | - |
dc.subject | Female | - |
dc.subject | Cricetinae | - |
dc.subject | Basic Helix-Loop-Helix Transcription Factors | - |
dc.subject | RUNX1 Translocation Partner 1 Protein | - |
dc.title | CBFA2T3-ZNF652 corepressor complex regulates transcription of the E-box gene HEB | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1074/jbc.M709136200 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Booker, G. [0000-0001-7207-4699] | - |
dc.identifier.orcid | Callen, D. [0000-0002-6189-9991] | - |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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