Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Pneumococcal Virulence Gene Expression and Host Cytokine Profiles during Pathogenesis of Invasive Disease
Author: Mahdi, L.
Ogunniyi, A.
Le Messurier, K.
Paton, J.
Citation: Infection and Immunity, 2008; 76(2):646-657
Publisher: Amer Soc Microbiology
Issue Date: 2008
ISSN: 0019-9567
Statement of
Layla K. Mahdi, Abiodun D. Ogunniyi, Kim S. LeMessurier, and James C. Paton
Abstract: Pneumococcal disease continues to account for significant morbidity and mortality worldwide. For the development of novel prophylactic and therapeutic strategies against the disease spectrum, a complete understanding of pneumococcal behavior in vivo is necessary. We evaluated the expression patterns of the proven and putative virulence factor genes adcR, cbpA, cbpD, cbpG, cpsA, nanA, pcpA, piaA, ply, psaA, pspA, and spxB after intranasal infection of CD1 mice with serotype 2, 4, and 6A pneumococci by real-time reverse transcription-PCR. Simultaneous gene expression patterns of selected host immunomodulatory molecules, CCL2, CCL5, CD54, CXCL2, interleukin-6, and tomor necrosis factor alpha, were also investigated. We show that pneumococcal virulence genes are differentially expressed in vivo, with some genes demonstrating niche- and serotype-specific differential expression. The in vivo expression patterns could not be attributed to in vitro differences in expression of the genes in transparent and opaque variants of the three strains. The host molecules were significantly upregulated, especially in the lungs, blood, and brains of mice. The pneumococcal-gene expression patterns support their ascribed roles in pathogenesis, providing insight into which protein combinations might be more appropriate as vaccine antigens against invasive disease. This is the first simultaneous comparison of bacterial- and host gene expression in the same animal during pathogenesis. The strategy provides a platform for prospective evaluation of interaction kinetics between invading pneumococci and human patients in culture-positive cases and should be feasible in other infection models.
Keywords: Lung
Nasal Cavity
Pneumococcal Infections
Bacterial Proteins
Virulence Factors
Colony Count, Microbial
Gene Expression Profiling
Reverse Transcriptase Polymerase Chain Reaction
Description: Copyright © 2008, American Society for Microbiology
DOI: 10.1128/IAI.01161-07
Appears in Collections:Aurora harvest 6
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.