Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/49945
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dc.contributor.authorFenech, M.en
dc.date.issued2007en
dc.identifier.citationNature Physics, 2007; 2(5):1084-1104en
dc.identifier.issn1745-2473en
dc.identifier.issn1750-2799en
dc.identifier.urihttp://hdl.handle.net/2440/49945-
dc.description© 2007 Nature Publishing Groupen
dc.description.abstractThe cytokinesis-block micronucleus cytome assay is a comprehensive system for measuring DNA damage, cytostasis and cytotoxicity. DNA damage events are scored specifically in once-divided binucleated (BN) cells and include (a) micronuclei (MNi), a biomarker of chromosome breakage and/or whole chromosome loss, (b) nucleoplasmic bridges (NPBs), a biomarker of DNA misrepair and/or telomere end-fusions, and (c) nuclear buds (NBUDs), a biomarker of elimination of amplified DNA and/or DNA repair complexes. Cytostatic effects are measured via the proportion of mono-, bi- and multinucleated cells and cytotoxicity via necrotic and/or apoptotic cell ratios. Further information regarding mechanisms leading to MNi, NPBs and NBUDs formation is obtained using centromere and/or telomere probes. The assay is being applied successfully for biomonitoring of in vivo genotoxin exposure, in vitro genotoxicity testing and in diverse research fields such as nutrigenomics and pharmacogenomics as well as a predictor of normal tissue and tumor radiation sensitivity and cancer risk. The procedure can take up to 5 days to completeen
dc.description.statementofresponsibilityMichael Fenechen
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.subjectDNA Damage; Biological Markers; Mutagens; Micronucleus Tests; Environmental Monitoring; Cytokinesis; Apoptosisen
dc.titleCytokinesis-block micronucleus cytome assayen
dc.typeJournal articleen
dc.identifier.rmid0020075025en
dc.identifier.doi10.1038/nprot.2007.77en
dc.identifier.pubid45992-
pubs.library.collectionPharmacology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidFenech, M. [0000-0002-8466-0991]en
Appears in Collections:Pharmacology publications

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