Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Potential roles of growth factor PDGF-BB in the bony repair of injured growth plate
Author: Chung, R.
Foster, B.
Zannettino, A.
Xian, C.
Citation: Bone, 2009; 44(5):878-885
Publisher: Elsevier Science Inc
Issue Date: 2009
ISSN: 8756-3282
Statement of
Rosa Chung, Bruce K. Foster, Andrew C.W. Zannettino and Cory J. Xian
Abstract: Injured growth plate cartilage is often repaired by bony tissue resulting in impaired bone growth in children. Using a rat injury model, our previous studies show that following the injury-induced initial inflammatory response, an influx of mesenchymal-like cells occurs within the growth plate injury site prior to formation of bony tissue. As platelet-derived growth factor (PDGF-BB) is a potent chemotactic factor of mesenchymal cells during skeletal tissue repair, we examined its role during the early fibrogenic response and the subsequent bony repair of injured growth plate. Following growth plate injury, rats received daily injection of the PDGF receptor (PDGFR) inhibitor, Imatinib, for 7 days. Immunohistochemical analysis of injured growth plate at day 1 showed the presence of PDGF-BB expression in some inflammatory cells, while at day 4 PDGFR was expressed by a proportion of the infiltrating mesenchymal cells at the injury site. By day 4, PDGFR inhibition reduced mesenchymal infiltrate (P<0.05); by day 14, Imatinib-treated rats exhibited less bony trabeculae and cartilaginous repair tissues, fewer osteoclasts and less bone marrow (BM) at the injury site, compared to vehicle controls (P<0.01). In vitro "scratch" migration assays with rat BM mesenchymal cells revealed that recombinant PDGF-BB increased cell migration into the "wound" (P<0.05), while Imatinib inhibited this chemotactic response. Quantitative RT-PCR analysis showed that Imatinib treatment decreased expression of the cartilage and bone related genes, Col2a1 and osteocalcin, respectively. These results suggest that PDGF-BB contributes to growth plate injury repair by promoting mesenchymal progenitor cell infiltration, the chondrogenic and osteogenic responses, and remodelling of the repair tissues.
Keywords: Growth Plate
Bone Marrow Cells
Stromal Cells
Mesenchymal Stem Cells
Rats, Sprague-Dawley
Collagen Type II
Receptor, Platelet-Derived Growth Factor beta
Platelet-Derived Growth Factor
Proto-Oncogene Proteins c-sis
Reverse Transcriptase Polymerase Chain Reaction
Cell Proliferation
Cell Movement
Gene Expression
Imatinib Mesylate
Salter-Harris Fractures
Description: Crown copyright © 2009 Published by Elsevier Inc.
DOI: 10.1016/j.bone.2009.01.377
Description (link):
Published version:
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.