Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/51670
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Platypus Pou5f1 reveals the first steps in the evolution of trophectoderm differentiation and pluripotency in mammals
Author: Niwa, H.
Sekita, Y.
Tsend-Ayush, E.
Grutzner, F.
Citation: Evolution & Development, 2008; 10(6):671-682
Publisher: Blackwell Publishing Inc
Issue Date: 2008
ISSN: 1520-541X
1525-142X
Statement of
Responsibility: 
Hitoshi Niwa, Yoko Sekita, Enkhjargal Tsend-Ayush and Frank Grützner
Abstract: SUMMARY Uterine nourishment of embryos by the placenta is a key feature of mammals. Although a variety of placenta types exist, they are all derived from the trophectoderm (TE) cell layer of the developing embryo. Egg-laying mammals (platypus and echidnas) are distinguished by a very short intrauterine embryo development, in which a simple placenta forms from TE-like cells. The Pou5f1 gene encodes a class V POU family transcription factor Oct3/4. In mice, Oct3/4 together with the highly conserved caudal-related homeobox transcription factor Cdx2, determines TE fate in pre-implantation development. In contrast to Cdx2, Pou5f1 has only been identified in eutherian mammals and marsupials, whereas, in other vertebrates, pou2 is considered to be the Pou5f1 ortholog. Here, we show that platypus and opossum genomes contain a Pou5f1 and pou2 homolog, pou2-related, indicating that these two genes are paralogues and arose by gene duplication in early mammalian evolution. In a complementation assay, we found that platypus or human Pou5f1, but not opossum or zebrafish pou2, restores self-renewal in Pou5f1-null mouse ES cells, showing that platypus possess a fully functional Pou5f1 gene. Interestingly, we discovered that parts of one of the conserved regions (CR4) is missing from the platypus Pou5f1 promoter, suggesting that the autoregulation and reciprocal inhibition between Pou5f1 and Cdx2 evolved after the divergence of monotremes and may be linked to the development of more elaborate placental types in marsupial and eutherian mammals.
Keywords: Ectoderm; Placenta; Animals; Mammals; Platypus; Humans; Mice; Sequence Alignment; Evolution, Molecular; Cell Differentiation; Amino Acid Sequence; Molecular Sequence Data; Female; POU Domain Factors; Octamer Transcription Factor-3; Embryonic Stem Cells; Promoter Regions, Genetic; Biological Evolution
Description: Journal compilation © 2009 Wiley Periodicals
RMID: 0020083398
DOI: 10.1111/j.1525-142X.2008.00280.x
Appears in Collections:Molecular and Biomedical Science publications
Environment Institute Leaders publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.