Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/54738
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | McDevitt, C. | - |
dc.contributor.author | Collins, R. | - |
dc.contributor.author | Conway, M. | - |
dc.contributor.author | Modok, S. | - |
dc.contributor.author | Storm, J. | - |
dc.contributor.author | Kerr, I. | - |
dc.contributor.author | Ford, R. | - |
dc.contributor.author | Callaghan, R. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Structure, 2006; 14(11):1623-1632 | - |
dc.identifier.issn | 0969-2126 | - |
dc.identifier.issn | 1878-4186 | - |
dc.identifier.uri | http://hdl.handle.net/2440/54738 | - |
dc.description.abstract | ABCG2 is a multidrug efflux pump associated with resistance of cancer cells to a plethora of unrelated drugs. ABCG2 is a “half-transporter,” and previous studies have indicated that it forms homodimers and higher oligomeric species. In this manuscript, electron microscopic structural analysis directly addressed this issue. An N-terminal hexahistidine-tagged ABCG2R482G isoform was expressed to high levels in insect cells. An extensive detergent screen was employed to effect extraction of ABCG2R482G from membranes and identified only the fos-choline detergents as efficient. Soluble protein was purified to >95% homogeneity by a three-step procedure while retaining the ability to bind substrates. Cryonegative stain electron microscopy of purified ABCG2R482G provided 3D structural data at a resolution of 18 Å. Single-particle analysis revealed that the complex forms a tetrameric complex (180 Å in diameter × 140 Å high) with an aqueous central region. We interpret the tetrameric structure as comprising four homodimeric ABCG2R482G complexes. | - |
dc.description.statementofresponsibility | Christopher A. McDevitt, Richard F. Collins, Michael Conway, Szabolcs Modok, Janet Storm, Ian D. Kerr, Robert C. Ford and Richard Callaghan | - |
dc.language.iso | en | - |
dc.publisher | Cell Press | - |
dc.source.uri | http://dx.doi.org/10.1016/j.str.2006.08.014 | - |
dc.subject | Cellbio | - |
dc.subject | Cellcycle | - |
dc.title | Purification and 3-D structural analysis of oligomeric human multidrug transporter ABCG2 | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.str.2006.08.014 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McDevitt, C. [0000-0003-1596-4841] | - |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.